Reassessment of Piwi Binding to the Genome and Piwi Impact on RNA Polymerase II Distribution

• Published in: Developmental cell Vol. 32; no. 6; pp. 772 - 774
• Main Authors: Lin, Haifan, Chen, Mengjie, Kundaje, Anshul, Valouev, Anton, Yin, Hang, Liu, Na, Neuenkirchen, Nils, Zhong, Mei, Snyder, Michael
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 23.03.2015
• Subjects: Animals; Argonaute Proteins - genetics; Base Sequence; Binding Sites - genetics; Chromatin Immunoprecipitation; Chromosomal Proteins, Non-Histone - genetics; DNA-Binding Proteins - genetics; Drosophila melanogaster; Drosophila Proteins - genetics; Genome; High-Throughput Nucleotide Sequencing; Histone-Lysine N-Methyltransferase - genetics; Methyltransferases - genetics; RNA Interference; RNA Polymerase II - genetics; RNA, Small Interfering - genetics; Sequence Analysis, DNA

Drosophila Piwi was reported by Huang et al. (2013) to be guided by piRNAs to piRNA-complementary sites in the genome, which then recruits heterochromatin protein 1a and histone methyltransferase Su(Var)3-9 to the sites. Among additional findings, Huang et al. (2013) also reported Piwi binding sites...