An immunoproteomic approach revealed antigenic proteins enhancing serodiagnosis performance of bird fancier's lung

• Published in: Journal of immunological methods Vol. 450; pp. 58 - 65
• Main Authors: Rouzet, Adeline, Reboux, Gabriel, Dalphin, Jean-Charles, Gondouin, Anne, De Vuyst, Paul, Balliau, Thierry, Millon, Laurence, Valot, Benoit, Roussel, Sandrine
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2017; Elsevier
• Subjects: 2D electrophoresis; Adult; Aged; Aged, 80 and over; Allergens - immunology; Animals; Area Under Curve; Avian Proteins - immunology; Biochemistry, Molecular Biology; biomarkers; Bird fancier's lung; Bird Fancier's Lung - blood; Bird Fancier's Lung - diagnosis; Bird Fancier's Lung - immunology; Birds - immunology; breathing; Case-Control Studies; Chromatography, Liquid; Ecology, environment; Electrophoresis, Agar Gel; Electrophoresis, Gel, Two-Dimensional; ELISA; Endopeptidases - immunology; Enzyme Precursors - immunology; Enzyme-Linked Immunosorbent Assay; Escherichia coli; Feces; Female; Genomics; Health; Human health and pathology; Humans; hypersensitivity; Hypersensitivity pneumonitis; immune system; immunoblotting; Immunoelectrophoresis; Immunoglobulin Light Chains, Surrogate - immunology; immunoglobulins; Inhalation Exposure; Life Sciences; Male; Middle Aged; patients; pigeons; pneumonia; Predictive Value of Tests; proteomics; Proteomics - methods; Pulmonology and respiratory tract; Recombinant antigens; recombinant proteins; Reproducibility of Results; ROC Curve; Serodiagnosis; Serologic Tests; Tandem Mass Spectrometry; DD; HVs; Hypersensitivity pneumonitis; Recombinant antigens; HP; BFL; AECs; 2-DE; PDE; LC-MS/MS; Bird fancier's lung; IEP; IGLL1; Serodiagnosis; 2D electrophoresis; ProE; ELISA
• ISSN: 0022-1759; 1872-7905; 1872-7905
• DOI: 10.1016/j.jim.2017.07.012

Bird fancier's lung (BFL) caused by repeated inhalation of avian proteins is the most common form of hypersensitivity pneumonitis. However, the exact identification of proteins involved is unknown, and serological test use for diagnosis need to be standardized. The objectives of this study were...