A proteomic analysis of green and white sturgeon larvae exposed to heat stress and selenium

• Published in: The Science of the total environment Vol. 408; no. 16; pp. 3176 - 3188
• Main Authors: Silvestre, Frédéric, Linares-Casenave, Javier, Doroshov, Serge I., Kültz, Dietmar
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 15.07.2010; [Amsterdam; New York]: Elsevier Science; Elsevier
• Subjects: Abundance; Animal, plant and microbial ecology; Animals; Applied ecology; Biological and medical sciences; Biomarker; Cluster Analysis; Ecotoxicology, biological effects of pollution; Electrophoresis, Gel, Two-Dimensional; Exposure; Fishes - growth & development; Fundamental and applied biological sciences. Psychology; Hot Temperature; Inhibitors; Kinases; Larva - drug effects; Larva - metabolism; Mortality; Proteins; Proteomics; Selenium; Selenium - toxicity; Species Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sturgeon; Techniques; Temperature; Thermoregulation. Hibernation. Estivation. Ecophysiology and environmental effects; Valosin-containing protein; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Water Pollutants, Chemical - toxicity; AAA ATPases; VCP; Temperature; Biomarker; SF Bay Delta; Se-L-Met; DPS; PPIase; STIP1; PDI; UPR; TPR; UPS; Selenium; WS; BCA; HSP; PR65; ST13; HCA; NCBInr; Sturgeon; PP2A; GS; ER; Se; CV; SR protein; Proteomics; HCCA; PEP; D36 and D45; L-Met; Valosin-containing protein; Biological marker; Environmental factor; Fry; Stress; Vertebrata; Pisces; Acipenser transmontanus; Water pollution
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2010.04.005

Temperature and selenium are two environmental parameters that potentially affect reproduction and stock recruitment of sturgeon in the San Francisco Bay/Delta Estuary. To identify proteins whose expression is modified by these environmental stressors, we performed a proteomic analysis on larval green and white sturgeons exposed to 18 or 26 °C and micro-injected with Seleno-L-Methionine to reach 8 µg g − 1 selenium body burden, with L-Methionine as a control. Selenium and high temperature induced mortalities and abnormal morphologies in both species, with a higher mortality in green sturgeon. Larval proteins were separated by two-dimensional gel electrophoresis and differential abundances were detected following spot quantitation and hierarchical cluster analysis. In green sturgeon, 34 of 551 protein spots detected on gels showed a variation in abundance whereas in white sturgeon only 9 of 580 protein spots were differentially expressed ( P < 0.01). Gel replicates were first grouped according to heat treatment. Fifteen of these spots were identified using MALDI TOF/TOF mass spectrometry. Proteins involved in protein folding, protein synthesis, protein degradation, ATP supply and structural proteins changed in abundance in response to heat and/or selenium. 40S ribosomal protein SA, FK506-binding protein 10, 65 kDa regulatory subunit A of protein phosphatase 2, protein disulfide isomerase, stress-induced-phosphoprotein 1, suppression of tumorigenicity 13 and collagen type II alpha 1, were differentially expressed in high temperature treatment only. Serine/arginine repetitive matrix protein 1, creatine kinase, serine peptidase inhibitor Kazal type 5 and HSP90 were sensitive to combined temperature and selenium exposure. Valosin-containing protein, a protein involved in aggresome formation and in protein quality control decreased more than 50% in response to selenium treatment. Potential use of such proteins as biomarkers of environmental stressors in larval sturgeons could indicate early warning signals preceding population decline.