Laser Capture and Deep Sequencing Reveals the Transcriptomic Programmes Regulating the Onset of Pancreas and Liver Differentiation in Human Embryos

• Published in: Stem cell reports Vol. 9; no. 5; pp. 1387 - 1394
• Main Authors: Jennings, Rachel E., Berry, Andrew A., Gerrard, David T., Wearne, Stephen J., Strutt, James, Withey, Sarah, Chhatriwala, Mariya, Piper Hanley, Karen, Vallier, Ludovic, Bobola, Nicoletta, Hanley, Neil A.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 14.11.2017; Elsevier
• Subjects: Cell Differentiation; development; embryo; Gene Expression Profiling; Gene Expression Regulation, Developmental; human; Human Embryonic Stem Cells - cytology; Human Embryonic Stem Cells - metabolism; Humans; liver; Liver - embryology; Liver - metabolism; pancreas; Pancreas - embryology; Pancreas - metabolism; RNA sequencing; stem cell; Transcription Factors - genetics; Transcription Factors - metabolism; Transcriptional Activation; Transcriptome; RNA sequencing; development; stem cell; liver; pancreas; transcriptome; embryo; human

To interrogate the alternative fates of pancreas and liver in the earliest stages of human organogenesis, we developed laser capture, RNA amplification, and computational analysis of deep sequencing. Pancreas-enriched gene expression was less conserved between human and mouse than for liver. The dor...