CLEC-2 is required for development and maintenance of lymph nodes
The importance of CLEC-2, a natural ligand/receptor for Gp38/Podoplanin, in the formation of the lymphatic vasculature has recently been demonstrated. As the development and maintenance of lymph nodes (LNs) is dependent on the formation of the lymphatic vasculature and the differentiation of Gp38/Po...
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Published in | Blood Vol. 123; no. 20; pp. 3200 - 3207 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
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United States
Elsevier Inc
15.05.2014
American Society of Hematology |
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Abstract | The importance of CLEC-2, a natural ligand/receptor for Gp38/Podoplanin, in the formation of the lymphatic vasculature has recently been demonstrated. As the development and maintenance of lymph nodes (LNs) is dependent on the formation of the lymphatic vasculature and the differentiation of Gp38/Podoplanin+ stromal cells, we investigated the role of CLEC-2 in lymphoneogenesis and LN homeostasis. Using constitutive Clec1b−/− mice, we showed that while CLEC-2 was not necessary for initiation of the LN anlage, it was required at late stages of development. Constitutive deletion of CLEC-2 induced a profound defect in lymphatic endothelial cell proliferation, resulting in lack of LNs at birth. In contrast, conditional deletion of CLEC-2 in the megakaryocyte/platelet lineage in Clec1bfl/flPF4-Cre mice led to the development of blood-filled LNs and fibrosis, in absence of a proliferative defect of the lymphatic endothelial compartment. This phenotype was also observed in chimeric mice reconstituted with Clec1bfl/flPF4-Cre bone marrow, indicating that CLEC-2 expression in platelets was required for LN integrity. We demonstrated that LNs of Clec1bfl/flPF4-Cre mice are able to sustain primary immune responses but show a defect in immune cell recirculation after repeated immunizations, thus suggesting CLEC-2 as target in chronic immune response.
•CLEC-2 is necessary for lymphatic cell proliferation and lymph node anlage persistence after birth.•Lack of CLEC-2 expression on megakaryocytes and platelets compromises lymph node integrity in adult life. |
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AbstractList | CLEC-2 is necessary for lymphatic cell proliferation and lymph node anlage persistence after birth. Lack of CLEC-2 expression on megakaryocytes and platelets compromises lymph node integrity in adult life. The importance of CLEC-2, a natural ligand/receptor for Gp38/Podoplanin, in the formation of the lymphatic vasculature has recently been demonstrated. As the development and maintenance of lymph nodes (LNs) is dependent on the formation of the lymphatic vasculature and the differentiation of Gp38/Podoplanin(+) stromal cells, we investigated the role of CLEC-2 in lymphoneogenesis and LN homeostasis. Using constitutive Clec1b(-/-) mice, we showed that while CLEC-2 was not necessary for initiation of the LN anlage, it was required at late stages of development. Constitutive deletion of CLEC-2 induced a profound defect in lymphatic endothelial cell proliferation, resulting in lack of LNs at birth. In contrast, conditional deletion of CLEC-2 in the megakaryocyte/platelet lineage in Clec1b(fl/fl)PF4-Cre mice led to the development of blood-filled LNs and fibrosis, in absence of a proliferative defect of the lymphatic endothelial compartment. This phenotype was also observed in chimeric mice reconstituted with Clec1b(fl/fl)PF4-Cre bone marrow, indicating that CLEC-2 expression in platelets was required for LN integrity. We demonstrated that LNs of Clec1b(fl/fl)PF4-Cre mice are able to sustain primary immune responses but show a defect in immune cell recirculation after repeated immunizations, thus suggesting CLEC-2 as target in chronic immune response. The importance of CLEC-2, a natural ligand/receptor for Gp38/Podoplanin, in the formation of the lymphatic vasculature has recently been demonstrated. As the development and maintenance of lymph nodes (LNs) is dependent on the formation of the lymphatic vasculature and the differentiation of Gp38/Podoplanin+ stromal cells, we investigated the role of CLEC-2 in lymphoneogenesis and LN homeostasis. Using constitutive Clec1b−/− mice, we showed that while CLEC-2 was not necessary for initiation of the LN anlage, it was required at late stages of development. Constitutive deletion of CLEC-2 induced a profound defect in lymphatic endothelial cell proliferation, resulting in lack of LNs at birth. In contrast, conditional deletion of CLEC-2 in the megakaryocyte/platelet lineage in Clec1bfl/flPF4-Cre mice led to the development of blood-filled LNs and fibrosis, in absence of a proliferative defect of the lymphatic endothelial compartment. This phenotype was also observed in chimeric mice reconstituted with Clec1bfl/flPF4-Cre bone marrow, indicating that CLEC-2 expression in platelets was required for LN integrity. We demonstrated that LNs of Clec1bfl/flPF4-Cre mice are able to sustain primary immune responses but show a defect in immune cell recirculation after repeated immunizations, thus suggesting CLEC-2 as target in chronic immune response. •CLEC-2 is necessary for lymphatic cell proliferation and lymph node anlage persistence after birth.•Lack of CLEC-2 expression on megakaryocytes and platelets compromises lymph node integrity in adult life. CLEC-2 is necessary for lymphatic cell proliferation and lymph node anlage persistence after birth. Lack of CLEC-2 expression on megakaryocytes and platelets compromises lymph node integrity in adult life. The importance of CLEC-2, a natural ligand/receptor for Gp38/Podoplanin, in the formation of the lymphatic vasculature has recently been demonstrated. As the development and maintenance of lymph nodes (LNs) is dependent on the formation of the lymphatic vasculature and the differentiation of Gp38/Podoplanin + stromal cells, we investigated the role of CLEC-2 in lymphoneogenesis and LN homeostasis. Using constitutive Clec1b −/− mice, we showed that while CLEC-2 was not necessary for initiation of the LN anlage, it was required at late stages of development. Constitutive deletion of CLEC-2 induced a profound defect in lymphatic endothelial cell proliferation, resulting in lack of LNs at birth. In contrast, conditional deletion of CLEC-2 in the megakaryocyte/platelet lineage in Clec1b fl/fl PF4-Cre mice led to the development of blood-filled LNs and fibrosis, in absence of a proliferative defect of the lymphatic endothelial compartment. This phenotype was also observed in chimeric mice reconstituted with Clec1b fl/fl PF4-Cre bone marrow, indicating that CLEC-2 expression in platelets was required for LN integrity. We demonstrated that LNs of Clec1b fl/fl PF4-Cre mice are able to sustain primary immune responses but show a defect in immune cell recirculation after repeated immunizations, thus suggesting CLEC-2 as target in chronic immune response. |
Author | Desanti, Guillaume E. Marriott, Clare L. Watson, Steve P. Withers, David R. Caamaño, Jorge H. Nayar, Saba Lowe, Kate Bénézech, Cécile Finney, Brenda A. Buckley, Christopher D. Barone, Francesca |
Author_xml | – sequence: 1 givenname: Cécile surname: Bénézech fullname: Bénézech, Cécile email: cbenezec@staffmail.ed.ac.uk organization: School of Immunity and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom – sequence: 2 givenname: Saba surname: Nayar fullname: Nayar, Saba organization: School of Immunity and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom – sequence: 3 givenname: Brenda A. surname: Finney fullname: Finney, Brenda A. organization: Centre for Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom – sequence: 4 givenname: David R. surname: Withers fullname: Withers, David R. organization: School of Immunity and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom – sequence: 5 givenname: Kate surname: Lowe fullname: Lowe, Kate organization: Centre for Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom – sequence: 6 givenname: Guillaume E. surname: Desanti fullname: Desanti, Guillaume E. organization: School of Immunity and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom – sequence: 7 givenname: Clare L. surname: Marriott fullname: Marriott, Clare L. organization: School of Immunity and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom – sequence: 8 givenname: Steve P. surname: Watson fullname: Watson, Steve P. organization: Centre for Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom – sequence: 9 givenname: Jorge H. surname: Caamaño fullname: Caamaño, Jorge H. organization: School of Immunity and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom – sequence: 10 givenname: Christopher D. surname: Buckley fullname: Buckley, Christopher D. organization: School of Immunity and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom – sequence: 11 givenname: Francesca surname: Barone fullname: Barone, Francesca email: f.barone@bham.ac.uk organization: School of Immunity and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom |
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Cites_doi | 10.1182/blood-2009-04-216069 10.1111/j.1538-7836.2011.04335.x 10.1182/blood-2012-07-443325 10.4049/jimmunol.0903113 10.1679/aohc.48.135 10.1038/nri2832 10.1016/j.immuni.2012.05.022 10.1182/blood-2011-09-380709 10.1242/dev.028456 10.1093/intimm/13.10.1243 10.1002/art.21062 10.1182/blood-2010-02-270876 10.1002/eji.1830171203 10.1161/ATVBAHA.112.300672 10.1016/S0092-8674(00)81511-1 10.1111/j.1749-6632.2002.tb04876.x 10.1093/emboj/cdg342 10.1074/jbc.M110.167502 10.4049/jimmunol.177.5.3369 10.1091/mbc.e10-06-0489 10.4049/jimmunol.0804324 10.1146/annurev.immunol.26.021607.090257 10.1182/blood-2012-05-428607 10.1182/blood-2010-04-278564 10.1146/annurev-immunol-031210-101357 10.1016/j.immuni.2012.06.010 10.1038/emboj.2012.340 10.1111/j.1600-065X.1997.tb00955.x 10.1172/JCI70422 10.1038/nature12501 10.1084/jem.20092642 |
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References | Bertozzi, Schmaier, Mericko (bib17) 2010; 116 Kaldjian, Gretz, Anderson, Shi, Shaw (bib1) 2001; 13 Séverin, Pollitt, Navarro-Nuñez (bib11) 2011; 286 Wigle, Oliver (bib29) 1999; 98 Ruddle, Akirav (bib6) 2009; 183 Hess, Rawnsley, Jakus (bib22) 2014; 124 Cueni, Chen, Zhang (bib23) 2010; 116 Uhrin, Zaujec, Breuss (bib19) 2010; 115 Bénézech, White, Mader (bib26) 2010; 184 Schacht, Ramirez, Hong (bib18) 2003; 22 van de Pavert, Mebius (bib7) 2010; 10 Herzog, Fu, Wilson (bib25) 2013; 502 Vondenhoff, van de Pavert, Dillard (bib9) 2009; 136 Zhi, Rauova, Hayes (bib14) 2013; 121 Roozendaal, Mebius (bib3) 2011; 29 Oliver, Harvey (bib8) 2002; 979 Liao, Ruddle (bib30) 2006; 177 Hendriks, Duijvestijn, Kraal (bib10) 1987; 17 Suzuki-Inoue, Inoue, Ozaki (bib15) 2011; 9 Yang, García-Verdugo, Soriano-Navarro (bib20) 2012; 120 Bénézech, Mader, Desanti (bib27) 2012; 37 Hoshi, Kamiya, Aijima, Yoshida, Endo (bib28) 1985; 48 Barone, Bombardieri, Manzo (bib32) 2005; 52 Hägerling, Pollmann, Andreas (bib21) 2013; 32 Acton, Astarita, Malhotra (bib24) 2012; 37 Gretz, Anderson, Shaw (bib2) 1997; 156 Bender, May, Lorenz (bib13) 2013; 33 Randall, Carragher, Rangel-Moreno (bib5) 2008; 26 Suzuki-Inoue (bib12) 2009; 50 Martín-Villar, Fernández-Muñoz, Parsons (bib31) 2010; 21 Fletcher, Lukacs-Kornek, Reynoso (bib4) 2010; 207 Finney, Schweighoffer, Navarro-Núñez (bib16) 2012; 119 24832934 - Blood. 2014 May 15;123(20):3057-8 Randall (2019111817031012700_B5) 2008; 26 Uhrin (2019111817031012700_B19) 2010; 115 Hess (2019111817031012700_B22) 2014; 124 Bénézech (2019111817031012700_B26) 2010; 184 Liao (2019111817031012700_B30) 2006; 177 Gretz (2019111817031012700_B2) 1997; 156 Séverin (2019111817031012700_B11) 2011; 286 Cueni (2019111817031012700_B23) 2010; 116 Fletcher (2019111817031012700_B4) 2010; 207 Hendriks (2019111817031012700_B10) 1987; 17 Ruddle (2019111817031012700_B6) 2009; 183 Barone (2019111817031012700_B32) 2005; 52 Acton (2019111817031012700_B24) 2012; 37 Suzuki-Inoue (2019111817031012700_B12) 2009; 50 Bertozzi (2019111817031012700_B17) 2010; 116 Kaldjian (2019111817031012700_B1) 2001; 13 Herzog (2019111817031012700_B25) 2013; 502 van de Pavert (2019111817031012700_B7) 2010; 10 Zhi (2019111817031012700_B14) 2013; 121 Suzuki-Inoue (2019111817031012700_B15) 2011; 9 Hoshi (2019111817031012700_B28) 1985; 48 Vondenhoff (2019111817031012700_B9) 2009; 136 Martín-Villar (2019111817031012700_B31) 2010; 21 Finney (2019111817031012700_B16) 2012; 119 Bénézech (2019111817031012700_B27) 2012; 37 Schacht (2019111817031012700_B18) 2003; 22 Oliver (2019111817031012700_B8) 2002; 979 Hägerling (2019111817031012700_B21) 2013; 32 Yang (2019111817031012700_B20) 2012; 120 Bender (2019111817031012700_B13) 2013; 33 Roozendaal (2019111817031012700_B3) 2011; 29 Wigle (2019111817031012700_B29) 1999; 98 |
References_xml | – volume: 116 start-page: 4376 year: 2010 end-page: 4384 ident: bib23 article-title: Podoplanin-Fc reduces lymphatic vessel formation in vitro and in vivo and causes disseminated intravascular coagulation when transgenically expressed in the skin. publication-title: Blood contributor: fullname: Zhang – volume: 48 start-page: 135 year: 1985 end-page: 148 ident: bib28 article-title: Histological observations on rat popliteal lymph nodes after blockage of their afferent lymphatics. publication-title: Arch Histol Jpn contributor: fullname: Endo – volume: 17 start-page: 1691 year: 1987 end-page: 1695 ident: bib10 article-title: Rapid decrease in lymphocyte adherence to high endothelial venules in lymph nodes deprived of afferent lymphatic vessels. publication-title: Eur J Immunol contributor: fullname: Kraal – volume: 22 start-page: 3546 year: 2003 end-page: 3556 ident: bib18 article-title: T1alpha/podoplanin deficiency disrupts normal lymphatic vasculature formation and causes lymphedema. publication-title: EMBO J contributor: fullname: Hong – volume: 98 start-page: 769 year: 1999 end-page: 778 ident: bib29 article-title: Prox1 function is required for the development of the murine lymphatic system. publication-title: Cell contributor: fullname: Oliver – volume: 13 start-page: 1243 year: 2001 end-page: 1253 ident: bib1 article-title: Spatial and molecular organization of lymph node T cell cortex: a labyrinthine cavity bounded by an epithelium-like monolayer of fibroblastic reticular cells anchored to basement membrane-like extracellular matrix. publication-title: Int Immunol contributor: fullname: Shaw – volume: 50 start-page: 389 year: 2009 end-page: 398 ident: bib12 article-title: CLEC-2, the novel platelet activation receptor and its internal ligand, podoplanin. publication-title: Rinsho Ketsueki contributor: fullname: Suzuki-Inoue – volume: 32 start-page: 629 year: 2013 end-page: 644 ident: bib21 article-title: A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy. publication-title: EMBO J contributor: fullname: Andreas – volume: 115 start-page: 3997 year: 2010 end-page: 4005 ident: bib19 article-title: Novel function for blood platelets and podoplanin in developmental separation of blood and lymphatic circulation. publication-title: Blood contributor: fullname: Breuss – volume: 29 start-page: 23 year: 2011 end-page: 43 ident: bib3 article-title: Stromal cell-immune cell interactions. publication-title: Annu Rev Immunol contributor: fullname: Mebius – volume: 124 start-page: 273 year: 2014 end-page: 284 ident: bib22 article-title: Platelets mediate lymphovenous hemostasis to maintain blood-lymphatic separation throughout life. publication-title: J Clin Invest contributor: fullname: Jakus – volume: 119 start-page: 1747 year: 2012 end-page: 1756 ident: bib16 article-title: CLEC-2 and Syk in the megakaryocytic/platelet lineage are essential for development. publication-title: Blood contributor: fullname: Navarro-Núñez – volume: 10 start-page: 664 year: 2010 end-page: 674 ident: bib7 article-title: New insights into the development of lymphoid tissues. publication-title: Nat Rev Immunol contributor: fullname: Mebius – volume: 116 start-page: 661 year: 2010 end-page: 670 ident: bib17 article-title: Platelets regulate lymphatic vascular development through CLEC-2-SLP-76 signaling. publication-title: Blood contributor: fullname: Mericko – volume: 156 start-page: 11 year: 1997 end-page: 24 ident: bib2 article-title: Cords, channels, corridors and conduits: critical architectural elements facilitating cell interactions in the lymph node cortex. publication-title: Immunol Rev contributor: fullname: Shaw – volume: 121 start-page: 1858 year: 2013 end-page: 1867 ident: bib14 article-title: Cooperative integrin/ITAM signaling in platelets enhances thrombus formation in vitro and in vivo. publication-title: Blood contributor: fullname: Hayes – volume: 52 start-page: 1773 year: 2005 end-page: 1784 ident: bib32 article-title: Association of CXCL13 and CCL21 expression with the progressive organization of lymphoid-like structures in Sjögren's syndrome. publication-title: Arthritis Rheum contributor: fullname: Manzo – volume: 184 start-page: 4521 year: 2010 end-page: 4530 ident: bib26 article-title: Ontogeny of stromal organizer cells during lymph node development. publication-title: J Immunol contributor: fullname: Mader – volume: 183 start-page: 2205 year: 2009 end-page: 2212 ident: bib6 article-title: Secondary lymphoid organs: responding to genetic and environmental cues in ontogeny and the immune response. publication-title: J Immunol contributor: fullname: Akirav – volume: 177 start-page: 3369 year: 2006 end-page: 3379 ident: bib30 article-title: Synchrony of high endothelial venules and lymphatic vessels revealed by immunization. publication-title: J Immunol contributor: fullname: Ruddle – volume: 26 start-page: 627 year: 2008 end-page: 650 ident: bib5 article-title: Development of secondary lymphoid organs. publication-title: Annu Rev Immunol contributor: fullname: Rangel-Moreno – volume: 120 start-page: 2340 year: 2012 end-page: 2348 ident: bib20 article-title: Lymphatic endothelial progenitors bud from the cardinal vein and intersomitic vessels in mammalian embryos. publication-title: Blood contributor: fullname: Soriano-Navarro – volume: 207 start-page: 689 year: 2010 end-page: 697 ident: bib4 article-title: Lymph node fibroblastic reticular cells directly present peripheral tissue antigen under steady-state and inflammatory conditions. publication-title: J Exp Med contributor: fullname: Reynoso – volume: 286 start-page: 4107 year: 2011 end-page: 4116 ident: bib11 article-title: Syk-dependent phosphorylation of CLEC-2: a novel mechanism of hem-immunoreceptor tyrosine-based activation motif signaling. publication-title: J Biol Chem contributor: fullname: Navarro-Nuñez – volume: 21 start-page: 4387 year: 2010 end-page: 4399 ident: bib31 article-title: Podoplanin associates with CD44 to promote directional cell migration. publication-title: Mol Biol Cell contributor: fullname: Parsons – volume: 979 start-page: 159 year: 2002 end-page: 165 ident: bib8 article-title: A stepwise model of the development of lymphatic vasculature. publication-title: Ann N Y Acad Sci contributor: fullname: Harvey – volume: 37 start-page: 276 year: 2012 end-page: 289 ident: bib24 article-title: Podoplanin-rich stromal networks induce dendritic cell motility via activation of the C-type lectin receptor CLEC-2. publication-title: Immunity contributor: fullname: Malhotra – volume: 33 start-page: 926 year: 2013 end-page: 934 ident: bib13 article-title: Combined in vivo depletion of glycoprotein VI and C-type lectin-like receptor 2 severely compromises hemostasis and abrogates arterial thrombosis in mice. publication-title: Arterioscler Thromb Vasc Biol contributor: fullname: Lorenz – volume: 502 start-page: 105 year: 2013 end-page: 109 ident: bib25 article-title: Podoplanin maintains high endothelial venule integrity by interacting with platelet CLEC-2. publication-title: Nature contributor: fullname: Wilson – volume: 37 start-page: 721 year: 2012 end-page: 734 ident: bib27 article-title: Lymphotoxin-β receptor signaling through NF-κB2-RelB pathway reprograms adipocyte precursors as lymph node stromal cells. publication-title: Immunity contributor: fullname: Desanti – volume: 9 start-page: 44 year: 2011 end-page: 55 ident: bib15 article-title: Novel platelet activation receptor CLEC-2: from discovery to prospects. publication-title: J Thromb Haemost contributor: fullname: Ozaki – volume: 136 start-page: 29 year: 2009 end-page: 34 ident: bib9 article-title: Lymph sacs are not required for the initiation of lymph node formation. publication-title: Development contributor: fullname: Dillard – volume: 115 start-page: 3997 issue: 19 year: 2010 ident: 2019111817031012700_B19 article-title: Novel function for blood platelets and podoplanin in developmental separation of blood and lymphatic circulation. publication-title: Blood doi: 10.1182/blood-2009-04-216069 contributor: fullname: Uhrin – volume: 9 start-page: 44 issue: suppl 1 year: 2011 ident: 2019111817031012700_B15 article-title: Novel platelet activation receptor CLEC-2: from discovery to prospects. publication-title: J Thromb Haemost doi: 10.1111/j.1538-7836.2011.04335.x contributor: fullname: Suzuki-Inoue – volume: 121 start-page: 1858 issue: 10 year: 2013 ident: 2019111817031012700_B14 article-title: Cooperative integrin/ITAM signaling in platelets enhances thrombus formation in vitro and in vivo. publication-title: Blood doi: 10.1182/blood-2012-07-443325 contributor: fullname: Zhi – volume: 184 start-page: 4521 issue: 8 year: 2010 ident: 2019111817031012700_B26 article-title: Ontogeny of stromal organizer cells during lymph node development. publication-title: J Immunol doi: 10.4049/jimmunol.0903113 contributor: fullname: Bénézech – volume: 48 start-page: 135 issue: 2 year: 1985 ident: 2019111817031012700_B28 article-title: Histological observations on rat popliteal lymph nodes after blockage of their afferent lymphatics. publication-title: Arch Histol Jpn doi: 10.1679/aohc.48.135 contributor: fullname: Hoshi – volume: 10 start-page: 664 issue: 9 year: 2010 ident: 2019111817031012700_B7 article-title: New insights into the development of lymphoid tissues. publication-title: Nat Rev Immunol doi: 10.1038/nri2832 contributor: fullname: van de Pavert – volume: 37 start-page: 276 issue: 2 year: 2012 ident: 2019111817031012700_B24 article-title: Podoplanin-rich stromal networks induce dendritic cell motility via activation of the C-type lectin receptor CLEC-2. publication-title: Immunity doi: 10.1016/j.immuni.2012.05.022 contributor: fullname: Acton – volume: 119 start-page: 1747 issue: 7 year: 2012 ident: 2019111817031012700_B16 article-title: CLEC-2 and Syk in the megakaryocytic/platelet lineage are essential for development. publication-title: Blood doi: 10.1182/blood-2011-09-380709 contributor: fullname: Finney – volume: 136 start-page: 29 issue: 1 year: 2009 ident: 2019111817031012700_B9 article-title: Lymph sacs are not required for the initiation of lymph node formation. publication-title: Development doi: 10.1242/dev.028456 contributor: fullname: Vondenhoff – volume: 13 start-page: 1243 issue: 10 year: 2001 ident: 2019111817031012700_B1 article-title: Spatial and molecular organization of lymph node T cell cortex: a labyrinthine cavity bounded by an epithelium-like monolayer of fibroblastic reticular cells anchored to basement membrane-like extracellular matrix. publication-title: Int Immunol doi: 10.1093/intimm/13.10.1243 contributor: fullname: Kaldjian – volume: 52 start-page: 1773 issue: 6 year: 2005 ident: 2019111817031012700_B32 article-title: Association of CXCL13 and CCL21 expression with the progressive organization of lymphoid-like structures in Sjögren’s syndrome. publication-title: Arthritis Rheum doi: 10.1002/art.21062 contributor: fullname: Barone – volume: 116 start-page: 661 issue: 4 year: 2010 ident: 2019111817031012700_B17 article-title: Platelets regulate lymphatic vascular development through CLEC-2-SLP-76 signaling. publication-title: Blood doi: 10.1182/blood-2010-02-270876 contributor: fullname: Bertozzi – volume: 17 start-page: 1691 issue: 12 year: 1987 ident: 2019111817031012700_B10 article-title: Rapid decrease in lymphocyte adherence to high endothelial venules in lymph nodes deprived of afferent lymphatic vessels. publication-title: Eur J Immunol doi: 10.1002/eji.1830171203 contributor: fullname: Hendriks – volume: 50 start-page: 389 issue: 5 year: 2009 ident: 2019111817031012700_B12 article-title: CLEC-2, the novel platelet activation receptor and its internal ligand, podoplanin. publication-title: Rinsho Ketsueki contributor: fullname: Suzuki-Inoue – volume: 33 start-page: 926 issue: 5 year: 2013 ident: 2019111817031012700_B13 article-title: Combined in vivo depletion of glycoprotein VI and C-type lectin-like receptor 2 severely compromises hemostasis and abrogates arterial thrombosis in mice. publication-title: Arterioscler Thromb Vasc Biol doi: 10.1161/ATVBAHA.112.300672 contributor: fullname: Bender – volume: 98 start-page: 769 issue: 6 year: 1999 ident: 2019111817031012700_B29 article-title: Prox1 function is required for the development of the murine lymphatic system. publication-title: Cell doi: 10.1016/S0092-8674(00)81511-1 contributor: fullname: Wigle – volume: 979 start-page: 159 year: 2002 ident: 2019111817031012700_B8 article-title: A stepwise model of the development of lymphatic vasculature. publication-title: Ann N Y Acad Sci doi: 10.1111/j.1749-6632.2002.tb04876.x contributor: fullname: Oliver – volume: 22 start-page: 3546 issue: 14 year: 2003 ident: 2019111817031012700_B18 article-title: T1alpha/podoplanin deficiency disrupts normal lymphatic vasculature formation and causes lymphedema. publication-title: EMBO J doi: 10.1093/emboj/cdg342 contributor: fullname: Schacht – volume: 286 start-page: 4107 issue: 6 year: 2011 ident: 2019111817031012700_B11 article-title: Syk-dependent phosphorylation of CLEC-2: a novel mechanism of hem-immunoreceptor tyrosine-based activation motif signaling. publication-title: J Biol Chem doi: 10.1074/jbc.M110.167502 contributor: fullname: Séverin – volume: 177 start-page: 3369 issue: 5 year: 2006 ident: 2019111817031012700_B30 article-title: Synchrony of high endothelial venules and lymphatic vessels revealed by immunization. publication-title: J Immunol doi: 10.4049/jimmunol.177.5.3369 contributor: fullname: Liao – volume: 21 start-page: 4387 issue: 24 year: 2010 ident: 2019111817031012700_B31 article-title: Podoplanin associates with CD44 to promote directional cell migration. publication-title: Mol Biol Cell doi: 10.1091/mbc.e10-06-0489 contributor: fullname: Martín-Villar – volume: 183 start-page: 2205 issue: 4 year: 2009 ident: 2019111817031012700_B6 article-title: Secondary lymphoid organs: responding to genetic and environmental cues in ontogeny and the immune response. publication-title: J Immunol doi: 10.4049/jimmunol.0804324 contributor: fullname: Ruddle – volume: 26 start-page: 627 year: 2008 ident: 2019111817031012700_B5 article-title: Development of secondary lymphoid organs. publication-title: Annu Rev Immunol doi: 10.1146/annurev.immunol.26.021607.090257 contributor: fullname: Randall – volume: 120 start-page: 2340 issue: 11 year: 2012 ident: 2019111817031012700_B20 article-title: Lymphatic endothelial progenitors bud from the cardinal vein and intersomitic vessels in mammalian embryos. publication-title: Blood doi: 10.1182/blood-2012-05-428607 contributor: fullname: Yang – volume: 116 start-page: 4376 issue: 20 year: 2010 ident: 2019111817031012700_B23 article-title: Podoplanin-Fc reduces lymphatic vessel formation in vitro and in vivo and causes disseminated intravascular coagulation when transgenically expressed in the skin. publication-title: Blood doi: 10.1182/blood-2010-04-278564 contributor: fullname: Cueni – volume: 29 start-page: 23 year: 2011 ident: 2019111817031012700_B3 article-title: Stromal cell-immune cell interactions. publication-title: Annu Rev Immunol doi: 10.1146/annurev-immunol-031210-101357 contributor: fullname: Roozendaal – volume: 37 start-page: 721 issue: 4 year: 2012 ident: 2019111817031012700_B27 article-title: Lymphotoxin-β receptor signaling through NF-κB2-RelB pathway reprograms adipocyte precursors as lymph node stromal cells. publication-title: Immunity doi: 10.1016/j.immuni.2012.06.010 contributor: fullname: Bénézech – volume: 32 start-page: 629 issue: 5 year: 2013 ident: 2019111817031012700_B21 article-title: A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy. publication-title: EMBO J doi: 10.1038/emboj.2012.340 contributor: fullname: Hägerling – volume: 156 start-page: 11 year: 1997 ident: 2019111817031012700_B2 article-title: Cords, channels, corridors and conduits: critical architectural elements facilitating cell interactions in the lymph node cortex. publication-title: Immunol Rev doi: 10.1111/j.1600-065X.1997.tb00955.x contributor: fullname: Gretz – volume: 124 start-page: 273 issue: 1 year: 2014 ident: 2019111817031012700_B22 article-title: Platelets mediate lymphovenous hemostasis to maintain blood-lymphatic separation throughout life. publication-title: J Clin Invest doi: 10.1172/JCI70422 contributor: fullname: Hess – volume: 502 start-page: 105 issue: 7469 year: 2013 ident: 2019111817031012700_B25 article-title: Podoplanin maintains high endothelial venule integrity by interacting with platelet CLEC-2. publication-title: Nature doi: 10.1038/nature12501 contributor: fullname: Herzog – volume: 207 start-page: 689 issue: 4 year: 2010 ident: 2019111817031012700_B4 article-title: Lymph node fibroblastic reticular cells directly present peripheral tissue antigen under steady-state and inflammatory conditions. publication-title: J Exp Med doi: 10.1084/jem.20092642 contributor: fullname: Fletcher |
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Snippet | The importance of CLEC-2, a natural ligand/receptor for Gp38/Podoplanin, in the formation of the lymphatic vasculature has recently been demonstrated. As the... CLEC-2 is necessary for lymphatic cell proliferation and lymph node anlage persistence after birth. Lack of CLEC-2 expression on megakaryocytes and platelets... CLEC-2 is necessary for lymphatic cell proliferation and lymph node anlage persistence after birth. Lack of CLEC-2 expression on megakaryocytes and platelets... |
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SubjectTerms | Animals Blood Platelets - metabolism Cell Proliferation Cells, Cultured Endothelium, Lymphatic - cytology Endothelium, Lymphatic - metabolism Gene Deletion Lectins, C-Type - genetics Lectins, C-Type - metabolism Lymph Nodes - cytology Lymph Nodes - growth & development Lymph Nodes - metabolism Lymphangiogenesis Megakaryocytes - metabolism Mice Mice, Inbred C57BL Vascular Biology |
Title | CLEC-2 is required for development and maintenance of lymph nodes |
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