Potent neutralization of SARS-CoV-2 variants of concern by an antibody with an uncommon genetic signature and structural mode of spike recognition

• Published in: Cell reports (Cambridge) Vol. 37; no. 1; p. 109784
• Main Authors: Kramer, Kevin J., Johnson, Nicole V., Shiakolas, Andrea R., Suryadevara, Naveenchandra, Periasamy, Sivakumar, Raju, Nagarajan, Williams, Jazmean K., Wrapp, Daniel, Zost, Seth J., Walker, Lauren M., Wall, Steven C., Holt, Clinton M., Hsieh, Ching-Lin, Sutton, Rachel E., Paulo, Ariana, Nargi, Rachel S., Davidson, Edgar, Doranz, Benjamin J., Crowe, James E., Bukreyev, Alexander, Carnahan, Robert H., McLellan, Jason S., Georgiev, Ivelin S.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 05.10.2021; The Author(s); Elsevier
• Subjects: Angiotensin-Converting Enzyme 2 - chemistry; Angiotensin-Converting Enzyme 2 - immunology; Animals; Antibodies, Monoclonal - immunology; Antibodies, Neutralizing - immunology; Antibodies, Viral - immunology; antibody discovery; Antibody Formation; Cell Line; Chlorocebus aethiops; COVID-19; COVID-19 - genetics; COVID-19 - immunology; COVID-19 - virology; cryo-EM; Cryoelectron Microscopy; Delta variant; Epitope Mapping - methods; Epitopes - chemistry; Epitopes - immunology; High-Throughput Screening Assays - methods; Humans; LIBRA-seq; Male; Middle Aged; monoclonal antibodies biology; Protein Binding; Protein Interaction Domains and Motifs; Receptors, Antigen, B-Cell - chemistry; Receptors, Antigen, B-Cell - immunology; SARS-CoV-2; SARS-CoV-2 - chemistry; SARS-CoV-2 - genetics; SARS-CoV-2 - immunology; Spike Glycoprotein, Coronavirus - chemistry; Spike Glycoprotein, Coronavirus - immunology; Vero Cells; COVID-19; SARS-CoV-2; monoclonal antibodies biology; antibody discovery; Delta variant; cryo-EM; LIBRA-seq

The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) lineages that are more transmissible and resistant to currently approved antibody therapies poses a considerable challenge to the clinical treatment of coronavirus disease (COVID-19). Therefore, the need for ongoing discov...