Cell-Type-Specific Adhesiveness and Proliferation Propensity on Laminin Isoforms Enable Purification of iPSC-Derived Corneal Epithelium

• Published in: Stem cell reports Vol. 14; no. 4; pp. 663 - 676
• Main Authors: Shibata, Shun, Hayashi, Ryuhei, Kudo, Yuji, Okubo, Toru, Imaizumi, Tsutomu, Katayama, Tomohiko, Ishikawa, Yuki, Kobayashi, Yuki, Toga, Junko, Taniguchi, Yukimasa, Honma, Yoichi, Sekiguchi, Kiyotoshi, Nishida, Kohji
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 14.04.2020; Elsevier
• Subjects: Cell Adhesion - drug effects; cell competition; Cell Differentiation - drug effects; Cell Proliferation - drug effects; cell purification; Cell Separation - methods; Cells, Cultured; corneal epithelial cells (CECs); Epithelial Cells - cytology; Epithelial Cells - drug effects; Epithelial Cells - metabolism; Epithelium, Corneal - cytology; human induced pluripotent stem cells (hiPSCs); Humans; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - drug effects; Integrins - metabolism; Laminin - pharmacology; laminin isoforms; Protein Isoforms - pharmacology; substrate-specific adhesion; substrate-specific adhesion; cell purification; cell competition; laminin isoforms; human induced pluripotent stem cells (hiPSCs); corneal epithelial cells (CECs)

A treatment for intractable diseases is expected to be the replacement of damaged tissues with products from human induced pluripotent stem cells (hiPSCs). Target cell purification is a critical step for realizing hiPSC-based therapy. Here, we found that hiPSC-derived ocular cell types exhibited uni...