Agonist-activated cobalt uptake identifies divalent cation—Permeable kainate receptors on neurons and glial cells

• Published in: Neuron (Cambridge, Mass.) Vol. 7; no. 3; pp. 509 - 518
• Main Authors: Pruss, Rebecca M., Akeson, Rachel L., Racke, Margaret M., Wilburn, Jennifer L.
• Format: Journal Article
• Language: English
• Published: Cambridge, MA Elsevier Inc 01.09.1991; Cell Press
• Subjects: Animals; Biological and medical sciences; Biological Transport; Calcium - metabolism; Cells, Cultured; Cerebellum - physiology; Cobalt - metabolism; Fundamental and applied biological sciences. Psychology; Glutamates - pharmacology; Hippocampus - physiology; In Vitro Techniques; Ion Channel Gating - drug effects; Ion Channels - physiology; Isolated neuron and nerve. Neuroglia; Kainic Acid - pharmacology; Manganese - metabolism; N-Methylaspartate - pharmacology; Neurons - physiology; Quisqualic Acid - pharmacology; Rats; Rats, Inbred Strains; Receptors, Kainic Acid; Receptors, Neurotransmitter - physiology; Vertebrates: nervous system and sense organs; Cerebellum; Immunohistochemistry; Cell culture; Glial cell; Rat; Rodentia; Central nervous system; Fluorescence; Glutamate receptor; Ionic current; Cobalt; Uptake; Kainate receptor; Vertebrata; Mammalia; Neuron; Divalent cation; Hippocampus; Brain (vertebrata)

Activation of kainate receptors causes Co 2+ influx into neurons, type-2 astrocytes, and O-2A progenitor cells. Agonist-activated Co 2+ uptake can be performed using cultured cells or fresh tissue slices. Based on the pattern of response to kainate, glutamate, and quisqualate, three functionally dif...