Lipid‐Peptide‐mRNA Nanoparticles Augment Radioiodine Uptake in Anaplastic Thyroid Cancer

• Published in: Advanced science Vol. 10; no. 3; pp. e2204334 - n/a
• Main Authors: Li, Qinglin, Zhang, Lizhuo, Lang, Jiayan, Tan, Zhuo, Feng, Qingqing, Zhu, Fei, Liu, Guangna, Ying, Zhangguo, Yu, Xuefei, Feng, He, Yi, Heqing, Wen, Qingliang, Jin, Tiefeng, Cheng, Keman, Zhao, Xiao, Ge, Minghua
• Format: Journal Article
• Language: English
• Published: Germany John Wiley & Sons, Inc 01.01.2023; John Wiley and Sons Inc; Wiley
• Subjects: Acids; anaplastic thyroid carcinoma; Animals; Cancer therapies; Cell Line, Tumor; Efficiency; Gene expression; Humans; Iodine; Iodine Radioisotopes - therapeutic use; Lipids; lipid‐peptide‐mRNA nanoparticles; Liposomes; Lymphatic system; Medical prognosis; Mice; Mice, Nude; mRNA delivery; Nanoparticles; Peptides; Protein expression; Proteins; RNA, Messenger; sodium iodide transporter; Statistical significance; Thyroid cancer; Thyroid Carcinoma, Anaplastic - genetics; Thyroid Carcinoma, Anaplastic - metabolism; Thyroid Carcinoma, Anaplastic - therapy; Thyroid Neoplasms - genetics; Thyroid Neoplasms - radiotherapy; Tumors; anaplastic thyroid carcinoma; lipid-peptide-mRNA nanoparticles; mRNA delivery; sodium iodide transporter
• ISSN: 2198-3844; 2198-3844
• DOI: 10.1002/advs.202204334

Restoring sodium iodide symporter (NIS) expression and function remains a major challenge for radioiodine therapy in anaplastic thyroid cancer (ATC). For more efficient delivery of messenger RNA (mRNA) to manipulate protein expression, a lipid‐peptide‐mRNA (LPm) nanoparticle (NP) is developed. The LPm NP is prepared by using amphiphilic peptides to assemble a peptide core and which is then coated with cationic lipids. An amphiphilic chimeric peptide, consisting of nine arginine and hydrophobic segments (6 histidine, C18 or cholesterol), is synthesized for adsorption of mRNA encoding NIS in RNase‐free conditions. In vitro studies show that LP(R9H6) m NP is most efficient at delivering mRNA and can increase NIS expression in ATC cells by more than 10‐fold. After intratumoral injection of NIS mRNA formulated in optimized LPm NP, NIS expression in subcutaneous ATC tumor tissue increases significantly in nude mice, resulting in more iodine 131 (131I) accumulation in the tumor, thereby significantly inhibiting tumor growth. Overall, this work designs three arginine‐rich peptide nanoparticles, contributing to the choice of liposome cores for gene delivery. LPm NP can serve as a promising adjunctive therapy for patients with ATC by restoring iodine affinity and enhancing the therapeutic efficacy of radioactive iodine. Lipid‐peptide‐mRNA (LPm) nanoparticles (NPs), is employed to deliver mRNA. mRNA encoding sodium iodide symporter (NIS) delivered by LPm NPs significantly augment the expression of NIS protein on the cell membrane of anaplastic thyroid cancer (ATC) in vivo and in vitro. NIS‐mRNA LPm NPs combining 131I therapy heightens intracellular aggregation of 131I and antitumor effect.