Site‐to‐Site Reproducibility and Spatial Resolution in MALDI–MSI of Peptides from Formalin‐Fixed Paraffin‐Embedded Samples

• Published in: Proteomics. Clinical applications Vol. 13; no. 1; pp. e1800029 - n/a
• Main Authors: Ly, Alice, Longuespée, Rémi, Casadonte, Rita, Wandernoth, Petra, Schwamborn, Kristina, Bollwein, Christine, Marsching, Christian, Kriegsmann, Katharina, Hopf, Carsten, Weichert, Wilko, Kriegsmann, Jörg, Schirmacher, Peter, Kriegsmann, Mark, Deininger, Sören‐Oliver
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.01.2019; John Wiley and Sons Inc
• Subjects: Animals; Data processing; Design of experiments; Digestion; Formaldehyde; formalin‐fixed paraffin embedded tissue; Humans; Hygroscopicity; Image processing; Image quality; Image segmentation; Intestine; Intestines - cytology; Ions; MALDI; Mice; Molecular Imaging; Ovarian Neoplasms - metabolism; Ovarian Neoplasms - pathology; Paraffin; Paraffin Embedding; Paraffins; Peptide Fragments - metabolism; Peptides; Proteomics - methods; Quality assessment; Reproducibility; Reproducibility of Results; Spatial data; Spatial discrimination; Spatial resolution; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Spraying; Teratoma; Teratoma - metabolism; Teratoma - pathology; Tissue Fixation; tissue typing; Trypsin; Workflow; tissue typing; formalin-fixed paraffin embedded tissue; workflow; MALDI; reproducibility

Purpose To facilitate the transition of MALDI–MS Imaging (MALDI–MSI) from basic science to clinical application, it is necessary to analyze formalin‐fixed paraffin‐embedded (FFPE) tissues. The aim is to improve in situ tryptic digestion for MALDI–MSI of FFPE samples and determine if similar results...