Single‐Nucleus RNA Sequencing and Spatial Transcriptomics Reveal the Immunological Microenvironment of Cervical Squamous Cell Carcinoma

• Published in: Advanced science Vol. 9; no. 29; pp. e2203040 - n/a
• Main Authors: Ou, Zhihua, Lin, Shitong, Qiu, Jiaying, Ding, Wencheng, Ren, Peidi, Chen, Dongsheng, Wang, Jiaxuan, Tong, Yihan, Wu, Di, Chen, Ao, Deng, Yuan, Cheng, Mengnan, Peng, Ting, Lu, Haorong, Yang, Huanming, Wang, Jian, Jin, Xin, Ma, Ding, Xu, Xun, Wang, Yanzhou, Li, Junhua, Wu, Peng
• Format: Journal Article
• Language: English
• Published: Germany John Wiley & Sons, Inc 01.10.2022; John Wiley and Sons Inc; Wiley
• Subjects: Apoptosis; Bar codes; Cancer therapies; cancer‐associated fibroblasts; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - metabolism; Carcinoma, Squamous Cell - pathology; Cervical cancer; Cervix; Chemotherapy; Female; Fibroblasts; Gene expression; Human papillomavirus; Humans; Immunology; Medical prognosis; Metastasis; Neoplasms, Connective Tissue; RNA, Small Nuclear; Sequence Analysis, RNA; single‐nucleus RNA sequencing; Smooth muscle; spatial transcriptomics; Transcriptome - genetics; tumor microenvironment; Tumor Microenvironment - genetics; Tumors; Uterine Cervical Neoplasms - genetics; Uterine Cervical Neoplasms - metabolism; Uterine Cervical Neoplasms - pathology; Womens health; cancer-associated fibroblasts; tumor microenvironment; single-nucleus RNA sequencing; cervical cancer; spatial transcriptomics

The effective treatment of advanced cervical cancer remains challenging. Herein, single‐nucleus RNA sequencing (snRNA‐seq) and SpaTial enhanced resolution omics‐sequencing (Stereo‐seq) are used to investigate the immunological microenvironment of cervical squamous cell carcinoma (CSCC). The expressi...