An Optogenetic‐Controlled Cell Reprogramming System for Driving Cell Fate and Light‐Responsive Chimeric Mice

• Published in: Advanced science Vol. 10; no. 4; pp. e2202858 - n/a
• Main Authors: Wang, Meiyan, Liu, Yuanxiao, Wang, Ziwei, Qiao, Longliang, Ma, Xiaoding, Hu, Lingfeng, Kong, Deqiang, Wang, Yuan, Ye, Haifeng
• Format: Journal Article
• Language: English
• Published: Germany John Wiley & Sons, Inc 01.02.2023; John Wiley and Sons Inc; Wiley
• Subjects: Animals; Cell Differentiation; cell reprogramming; Cellular Reprogramming - genetics; chimeric mice; CRISPR; endogenous transcription factors; Fibroblasts; Fibroblasts - metabolism; Gene expression; Gene loci; Genetic engineering; Genomes; Humans; Induced Pluripotent Stem Cells - metabolism; Light emitting diodes; Mice; Optogenetics; Plasmids; Proteins; regenerative medicines; Stem cells; endogenous transcription factors; cell reprogramming; chimeric mice; regenerative medicines; optogenetics

Pluripotent stem cells (PSCs) hold great promise for cell‐based therapies, disease modeling, and drug discovery. Classic somatic cell reprogramming to generate induced pluripotent stem cells (iPSCs) is often achieved based on overexpression of transcription factors (TFs). However, this process is li...