Development of marker-free transgenic Jatropha curcas producing curcin-deficient seeds through endosperm-specific RNAi-mediated gene silencing

• Published in: BMC plant biology Vol. 15; no. 1; p. 242
• Main Authors: Gu, Keyu, Tian, Dongsheng, Mao, Huizhu, Wu, Lifang, Yin, Zhongchao
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 08.10.2015; BioMed Central
• Subjects: Blotting, Northern; Blotting, Southern; Blotting, Western; DNA, Bacterial - genetics; Endosperm - genetics; Gene Expression Regulation, Plant; Genetic aspects; Genetic Markers; Genetically modified plants; Growth; Jatropha - genetics; Methods; Organ Specificity - genetics; Plants, Genetically Modified; Polymerase Chain Reaction; Ribosome Inactivating Proteins, Type 1 - biosynthesis; RNA Interference; RNA, Messenger - genetics; RNA, Messenger - metabolism; Seeds - genetics

Jatropha curcas L. is a potential biofuel plant and its seed oil is suitable for biodiesel production. Despite this promising application, jatropha seeds contain two major toxic components, namely phorbol esters and curcins. These compounds would reduce commercial value of seed cake and raise safety...