Detection of Aminoglycoside Resistant Bacteria in Sludge Samples From Norwegian Drinking Water Treatment Plants
Through a culture-based approach using sludge from drinking water treatment plants, this study reports on the presence of aminoglycoside resistant bacteria at 23 different geographical locations in Norway. Sludge samples are derived from a large environmental area including drinking water sources an...
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Published in | Frontiers in microbiology Vol. 10; p. 487 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
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Abstract | Through a culture-based approach using sludge from drinking water treatment plants, this study reports on the presence of aminoglycoside resistant bacteria at 23 different geographical locations in Norway. Sludge samples are derived from a large environmental area including drinking water sources and their surrounding catchment areas. Aminoglycoside resistant bacteria were detected at 18 of the sample sites. Only five samples did not show any growth of isolates resistant to the selected aminoglycosides, kanamycin and gentamycin. There was a statistically significant correlation between the numbers of kanamycin and gentamycin resistant bacteria isolated from the 23 samples, perhaps suggesting common determinants of resistance. Based on 16S rRNA sequencing of 223 aminoglycoside resistant isolates, three different genera of
were found to dominate across samples. These were
and
Further phenotypic and genotypic analyses showed that efflux pumps, reduced membrane permeability and four assayed genes coding for aminoglycoside modifying enzymes AAC(6')-Ib, AAC(3')-II, APH(3')-II, APH(3')-III, could only explain the resistance of a few of the isolates selected for testing.
'
was detected in 1.6% of total isolates,
'
and
'
in 0.8%, while
'
was not detected in any of the isolates. The isolates, for which potential resistance mechanisms were found, represented 13 different genera suggesting that aminoglycoside resistance is widespread in bacterial genera indigenous to sludge. The present study suggests that aminoglycoside resistant bacteria are present in Norwegian environments with limited anthropogenic exposures. However, the resistance mechanisms remain largely unknown, and further analyses, including culture-independent methods, could be performed to investigate other potential resistance mechanisms. This is, to our knowledge, the first large scale nationwide investigation of aminoglycoside resistance in the Norwegian environment. |
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AbstractList | Through a culture-based approach using sludge from drinking water treatment plants, this study reports on the presence of aminoglycoside resistant bacteria at 23 different geographical locations in Norway. Sludge samples are derived from a large environmental area including drinking water sources and their surrounding catchment areas. Aminoglycoside resistant bacteria were detected at 18 of the sample sites. Only five samples did not show any growth of isolates resistant to the selected aminoglycosides, kanamycin and gentamycin. There was a statistically significant correlation between the numbers of kanamycin and gentamycin resistant bacteria isolated from the 23 samples, perhaps suggesting common determinants of resistance. Based on 16S rRNA sequencing of 223 aminoglycoside resistant isolates, three different genera of Bacteroidetes were found to dominate across samples. These were Flavobacterium, Mucilaginibacter and Pedobacter. Further phenotypic and genotypic analyses showed that efflux pumps, reduced membrane permeability and four assayed genes coding for aminoglycoside modifying enzymes AAC(6′)-Ib, AAC(3′)-II, APH(3′)-II, APH(3′)-III, could only explain the resistance of a few of the isolates selected for testing. aph(3′)-II was detected in 1.6% of total isolates, aac(6′)-Ib and aph(3′)-III in 0.8%, while aac(3′)-II was not detected in any of the isolates. The isolates, for which potential resistance mechanisms were found, represented 13 different genera suggesting that aminoglycoside resistance is widespread in bacterial genera indigenous to sludge. The present study suggests that aminoglycoside resistant bacteria are present in Norwegian environments with limited anthropogenic exposures. However, the resistance mechanisms remain largely unknown, and further analyses, including culture-independent methods, could be performed to investigate other potential resistance mechanisms. This is, to our knowledge, the first large scale nationwide investigation of aminoglycoside resistance in the Norwegian environment. Through a culture-based approach using sludge from drinking water treatment plants, this study reports on the presence of aminoglycoside resistant bacteria at 23 different geographical locations in Norway. Sludge samples are derived from a large environmental area including drinking water sources and their surrounding catchment areas. Aminoglycoside resistant bacteria were detected at 18 of the sample sites. Only five samples did not show any growth of isolates resistant to the selected aminoglycosides, kanamycin and gentamycin. There was a statistically significant correlation between the numbers of kanamycin and gentamycin resistant bacteria isolated from the 23 samples, perhaps suggesting common determinants of resistance. Based on 16S rRNA sequencing of 223 aminoglycoside resistant isolates, three different genera of were found to dominate across samples. These were and Further phenotypic and genotypic analyses showed that efflux pumps, reduced membrane permeability and four assayed genes coding for aminoglycoside modifying enzymes AAC(6')-Ib, AAC(3')-II, APH(3')-II, APH(3')-III, could only explain the resistance of a few of the isolates selected for testing. ' was detected in 1.6% of total isolates, ' and ' in 0.8%, while ' was not detected in any of the isolates. The isolates, for which potential resistance mechanisms were found, represented 13 different genera suggesting that aminoglycoside resistance is widespread in bacterial genera indigenous to sludge. The present study suggests that aminoglycoside resistant bacteria are present in Norwegian environments with limited anthropogenic exposures. However, the resistance mechanisms remain largely unknown, and further analyses, including culture-independent methods, could be performed to investigate other potential resistance mechanisms. This is, to our knowledge, the first large scale nationwide investigation of aminoglycoside resistance in the Norwegian environment. Through a culture-based approach using sludge from drinking water treatment plants, this study reports on the presence of aminoglycoside resistant bacteria at 23 different geographical locations in Norway. Sludge samples are derived from a large environmental area including drinking water sources and their surrounding catchment areas. Aminoglycoside resistant bacteria were detected at 18 of the sample sites. Only five samples did not show any growth of isolates resistant to the selected aminoglycosides, kanamycin and gentamycin. There was a statistically significant correlation between the numbers of kanamycin and gentamycin resistant bacteria isolated from the 23 samples, perhaps suggesting common determinants of resistance. Based on 16S rRNA sequencing of 223 aminoglycoside resistant isolates, three different genera of Bacteroidetes were found to dominate across samples. These were Flavobacterium, Mucilaginibacter and Pedobacter. Further phenotypic and genotypic analyses showed that efflux pumps, reduced membrane permeability and four assayed genes coding for aminoglycoside modifying enzymes AAC(6′)-Ib, AAC(3′)-II, APH(3′)-II, APH(3′)-III, could only explain the resistance of a few of the isolates selected for testing. aph(3 ′ )-II was detected in 1.6% of total isolates, aac(6 ′ )-Ib and aph(3 ′ )-III in 0.8%, while aac(3 ′ )-II was not detected in any of the isolates. The isolates, for which potential resistance mechanisms were found, represented 13 different genera suggesting that aminoglycoside resistance is widespread in bacterial genera indigenous to sludge. The present study suggests that aminoglycoside resistant bacteria are present in Norwegian environments with limited anthropogenic exposures. However, the resistance mechanisms remain largely unknown, and further analyses, including culture-independent methods, could be performed to investigate other potential resistance mechanisms. This is, to our knowledge, the first large scale nationwide investigation of aminoglycoside resistance in the Norwegian environment. |
Author | Nielsen, Kaare Magne Charnock, Colin Ullmann, Ingvild F Lund, Vidar Tunsjø, Hege S Andreassen, Monica |
AuthorAffiliation | 1 Department of Life Sciences and Health, OsloMet – Oslo Metropolitan University , Oslo , Norway 2 Department of Zoonotic, Food- and Waterborne Infections, Norwegian Institute of Public Health , Oslo , Norway |
AuthorAffiliation_xml | – name: 2 Department of Zoonotic, Food- and Waterborne Infections, Norwegian Institute of Public Health , Oslo , Norway – name: 1 Department of Life Sciences and Health, OsloMet – Oslo Metropolitan University , Oslo , Norway |
Author_xml | – sequence: 1 givenname: Ingvild F surname: Ullmann fullname: Ullmann, Ingvild F organization: Department of Life Sciences and Health, OsloMet - Oslo Metropolitan University, Oslo, Norway – sequence: 2 givenname: Hege S surname: Tunsjø fullname: Tunsjø, Hege S organization: Department of Life Sciences and Health, OsloMet - Oslo Metropolitan University, Oslo, Norway – sequence: 3 givenname: Monica surname: Andreassen fullname: Andreassen, Monica organization: Department of Zoonotic, Food- and Waterborne Infections, Norwegian Institute of Public Health, Oslo, Norway – sequence: 4 givenname: Kaare Magne surname: Nielsen fullname: Nielsen, Kaare Magne organization: Department of Life Sciences and Health, OsloMet - Oslo Metropolitan University, Oslo, Norway – sequence: 5 givenname: Vidar surname: Lund fullname: Lund, Vidar organization: Department of Zoonotic, Food- and Waterborne Infections, Norwegian Institute of Public Health, Oslo, Norway – sequence: 6 givenname: Colin surname: Charnock fullname: Charnock, Colin organization: Department of Life Sciences and Health, OsloMet - Oslo Metropolitan University, Oslo, Norway |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/30918503$$D View this record in MEDLINE/PubMed |
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Cites_doi | 10.1080/07352689.2012.683373 10.1261/rna.068460.118 10.1111/1574-6976.12062 10.1128/AAC.00457-07 10.1016/0378-1119(82)90023-3 10.1089/wound.2014.0577 10.1016/j.scitotenv.2016.04.062 10.1371/journal.pone.0001800 10.1101/cshperspect.a027029 10.1128/AAC.47.8.2565-2571.2003 10.1093/emboj/21.10.2323 10.3389/fmicb.2017.00228 10.1016/j.envpol.2018.06.047 10.1039/C5MD00344J 10.1093/bioinformatics/bts252 10.1016/j.envpol.2015.07.028 10.1099/jmm.0.065789-0 10.1128/AEM.00142-06 10.1016/j.diagmicrobio.2013.10.001 10.3389/fmicb.2012.00408 10.1093/bioinformatics/btu033 10.1128/AAC.46.5.1402-1409.2002 10.1186/1471-2180-13-58 10.1016/j.idc.2016.02.011 10.1016/j.envpol.2009.05.051 10.1128/CMR.16.3.430-450.2003 10.1128/AAC.42.7.1778 10.1016/j.jbiotec.2003.10.011 10.1371/journal.pone.0017224 10.1126/science.1123061 10.1128/CMR.00043-12 10.1007/s00018-007-7034-x 10.1128/mBio.01172-17 10.1021/cb3005116 10.1128/AEM.69.8.4455-4462.2003 10.1016/j.envint.2018.04.041 |
ContentType | Journal Article |
Copyright | Copyright © 2019 Ullmann, Tunsjø, Andreassen, Nielsen, Lund and Charnock. 2019 Ullmann, Tunsjø, Andreassen, Nielsen, Lund and Charnock |
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Keywords | agriculture environmental bacteria sludge water treatment drinking water antimicrobial resistance (AMR) |
Language | English |
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Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Shauna Leanne Reckseidler-Zenteno, Athabasca University, Canada; Takashi Azuma, Osaka University of Pharmaceutical Sciences, Japan; Jana Jass, Örebro University, Sweden Edited by: Yuji Morita, Meiji Pharmaceutical University, Japan This article was submitted to Antimicrobials, Resistance and Chemotherapy, a section of the journal Frontiers in Microbiology |
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References | de Vries (B7) 2003; 69 Krishnamoorthy (B20) 2017; 8 Woegerbauer (B44) 2014; 63 Fernandez (B9) 2012; 25 Haldorsen (B16) 2014; 78 Tolmasky (B38) 2000; 5 Vaz-Moreira (B40) 2014; 38 Galimand (B13) 2003; 47 Martinez (B23) 2009; 157 Woegerbauer (B43) 2015; 206 Soler Bistue (B35) 2008; 3 Becker (B3) 2013; 8 Gad (B12) 2011; 6 Morita (B25) 1998; 42 Hermann (B17) 2007; 64 Narciso-da-Rocha (B27) 2016 Yokoyama (B46) 2003; 362 Miki (B24) 2004; 107 Pruesse (B31) 2012; 28 Morita (B26) 2012; 3 Viana (B41) 2018; 241 Rosellini (B34) 2012; 31 Ciccarelli (B5) 2006; 311 Hu (B18) 2013; 13 Ramirez (B33) 2010; 13 Tanner (B37) 1998; 64 Golebiewski (B15) 2007; 51 Lane (B21) 1991 Nordgård (B28) 2016 Beck (B2) 1982; 19 Stamatakis (B36) 2014; 30 Centron (B4) 2002; 46 Osei Sekyere (B30) 2017; 8 Krause (B19) 2016; 6 Doi (B8) 2016; 30 (B42) 2015 Larsson (B22) 2018; 117 (B29) 2016 (B32) 2017 Alakomi (B1) 2006; 72 (B6) 2017 Fong (B11) 2002; 21 Garneau-Tsodikova (B14) 2016; 7 Ying (B45) 2018; 25 Finnegan (B10) 2015; 4 Vakulenko (B39) 2003; 16 |
References_xml | – year: 1991 ident: B21 publication-title: Nucleic Acid Techniques in Bacterial Systematic. contributor: fullname: Lane – volume: 31 start-page: 401 year: 2012 ident: B34 article-title: Selectable markers and reporter genes: a well furnished toolbox for plant science and genetic engineering. publication-title: Crit. Rev. Plant Sci. doi: 10.1080/07352689.2012.683373 contributor: fullname: Rosellini – volume: 25 start-page: 247 year: 2018 ident: B45 article-title: Roles of specific aminoglycoside-ribosome interactions in the inhibition of translation. publication-title: RNA doi: 10.1261/rna.068460.118 contributor: fullname: Ying – year: 2015 ident: B42 publication-title: Global Action Plan on Antimicrobial Resistance. doi: 10.1111/1574-6976.12062 – volume: 51 start-page: 3789 year: 2007 ident: B15 article-title: Complete nucleotide sequence of the pCTX-M3 plasmid and its involvement in spread of the extended-spectrum beta-lactamase gene blaCTX-M-3. publication-title: Antimicrob Agents Chemother. doi: 10.1128/AAC.00457-07 contributor: fullname: Golebiewski – volume: 19 start-page: 327 year: 1982 ident: B2 article-title: Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5. publication-title: Gene doi: 10.1016/0378-1119(82)90023-3 contributor: fullname: Beck – volume: 4 start-page: 415 year: 2015 ident: B10 article-title: EDTA: an antimicrobial and antibiofilm agent for use in wound care. publication-title: Adv. Wound Care doi: 10.1089/wound.2014.0577 contributor: fullname: Finnegan – year: 2016 ident: B29 publication-title: Usage of Antimicrobial Agents and Occurrence of Antimicrobial Resistance in Norway. doi: 10.1016/j.scitotenv.2016.04.062 – volume: 3 year: 2008 ident: B35 article-title: Klebsiella pneumoniae multiresistance plasmid pMET1: similarity with the Yersinia pestis plasmid pCRY and integrative conjugative elements. publication-title: PLoS One doi: 10.1371/journal.pone.0001800 contributor: fullname: Soler Bistue – volume: 6 year: 2016 ident: B19 article-title: Aminoglycosides: an overview. publication-title: Cold Spring Harb. Perspect. Med. doi: 10.1101/cshperspect.a027029 contributor: fullname: Krause – volume: 38 start-page: 761 year: 2014 ident: B40 article-title: Bacterial diversity and antibiotic resistance in water habitats: searching the links with the human microbiome. publication-title: FEMS Microbiol. Rev. doi: 10.1111/1574-6976.12062 contributor: fullname: Vaz-Moreira – year: 2017 ident: B6 publication-title: Performance Standards for Antimicrobial Susceptibility Testing. – volume: 47 start-page: 2565 year: 2003 ident: B13 article-title: Plasmid-mediated high-level resistance to aminoglycosides in Enterobacteriaceae due to 16S rRNA methylation. publication-title: Antimicrob. Agents Chemother. doi: 10.1128/AAC.47.8.2565-2571.2003 contributor: fullname: Galimand – volume: 64 start-page: 3110 year: 1998 ident: B37 article-title: Specific ribosomal DNA sequences from diverse environmental settings correlate with experimental contaminants. publication-title: Appl. Environ. Microbiol. doi: 10.1371/journal.pone.0001800 contributor: fullname: Tanner – volume: 21 start-page: 2323 year: 2002 ident: B11 article-title: Substrate promiscuity of an aminoglycoside antibiotic resistance enzyme via target mimicry. publication-title: EMBO J. doi: 10.1093/emboj/21.10.2323 contributor: fullname: Fong – year: 2017 ident: B32 publication-title: R: A Language and Environment for Statistical Computing. doi: 10.3389/fmicb.2017.00228 – volume: 241 start-page: 1048 year: 2018 ident: B41 article-title: Environmental superbugs: the case study of Pedobacter spp. publication-title: Environ. Pollut. doi: 10.1016/j.envpol.2018.06.047 contributor: fullname: Viana – volume: 7 start-page: 11 year: 2016 ident: B14 article-title: Mechanisms of resistance to aminoglycoside antibiotics: overview and perspectives. publication-title: Medchemcomm doi: 10.1039/C5MD00344J contributor: fullname: Garneau-Tsodikova – volume: 13 start-page: 151 year: 2010 ident: B33 article-title: Aminoglycoside modifying enzymes. publication-title: Drug Resist. Updat. doi: 10.1093/bioinformatics/bts252 contributor: fullname: Ramirez – volume: 206 start-page: 342 year: 2015 ident: B43 article-title: Antibiotic resistance marker genes as environmental pollutants in GMO-pristine agricultural soils in Austria. publication-title: Environ. Pollut. doi: 10.1016/j.envpol.2015.07.028 contributor: fullname: Woegerbauer – volume: 63 start-page: 210 year: 2014 ident: B44 article-title: Prevalence of the aminoglycoside phosphotransferase genes aph(3’)-IIIa and aph(3’)-IIa in Escherichia coli, Enterococcus faecalis, Enterococcus faecium, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica and Staphylococcus aureus isolates in Austria. publication-title: J. Med. Microbiol. doi: 10.1099/jmm.0.065789-0 contributor: fullname: Woegerbauer – volume: 72 start-page: 4695 year: 2006 ident: B1 article-title: Weakening effect of cell permeabilizers on gram-negative bacteria causing biodeterioration. publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.00142-06 contributor: fullname: Alakomi – volume: 78 start-page: 66 year: 2014 ident: B16 article-title: Increased prevalence of aminoglycoside resistance in clinical isolates of Escherichia coli and Klebsiella spp. in Norway is associated with the acquisition of AAC(3)-II and AAC(6)-Ib. publication-title: Diagn. Microbiol. Infect. Dis. doi: 10.1016/j.diagmicrobio.2013.10.001 contributor: fullname: Haldorsen – volume: 3 year: 2012 ident: B26 article-title: MexXY multidrug efflux system of Pseudomonas aeruginosa. publication-title: Front. Microbiol. doi: 10.3389/fmicb.2012.00408 contributor: fullname: Morita – volume: 30 start-page: 1312 year: 2014 ident: B36 article-title: RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. publication-title: Bioinformatics doi: 10.1093/bioinformatics/btu033 contributor: fullname: Stamatakis – volume: 46 start-page: 1402 year: 2002 ident: B4 article-title: Presence of a group II intron in a multiresistant Serratia marcescens strain that harbors three integrons and a novel gene fusion. publication-title: Antimicrob. Agents Chemother. doi: 10.1128/AAC.46.5.1402-1409.2002 contributor: fullname: Centron – volume: 13 year: 2013 ident: B18 article-title: A high throughput multiplex PCR assay for simultaneous detection of seven aminoglycoside-resistance genes in Enterobacteriaceae. publication-title: BMC Microbiol. doi: 10.1186/1471-2180-13-58 contributor: fullname: Hu – volume: 8 year: 2017 ident: B30 article-title: Carbonyl cyanide m-chlorophenylhydrazine (CCCP) reverses resistance to colistin, but not to carbapenems and tigecycline in multidrug-resistant Enterobacteriaceae. publication-title: Front. Microbiol. doi: 10.3389/fmicb.2017.00228 contributor: fullname: Osei Sekyere – volume: 30 start-page: 523 year: 2016 ident: B8 article-title: Aminoglycoside resistance: the emergence of acquired 16S ribosomal RNA methyltransferases. publication-title: Infect. Dis. Clin. North Am. doi: 10.1016/j.idc.2016.02.011 contributor: fullname: Doi – volume: 362 start-page: 1888 year: 2003 ident: B46 article-title: Acquisition of 16S rRNA methylase gene in Pseudomonas aeruginosa. publication-title: Lancet doi: 10.1099/jmm.0.065789-0 contributor: fullname: Yokoyama – volume: 157 start-page: 2893 year: 2009 ident: B23 article-title: Environmental pollution by antibiotics and by antibiotic resistance determinants. publication-title: Environ. Pollut. doi: 10.1016/j.envpol.2009.05.051 contributor: fullname: Martinez – volume: 16 start-page: 430 year: 2003 ident: B39 article-title: Versatility of aminoglycosides and prospects for their future. publication-title: Clin. Microbiol. Rev. doi: 10.1128/CMR.16.3.430-450.2003 contributor: fullname: Vakulenko – volume: 42 start-page: 1778 year: 1998 ident: B25 article-title: NorM, a putative multidrug efflux protein, of Vibrio parahaemolyticus and its homolog in Escherichia coli. publication-title: Antimicrob. Agents Chemother. doi: 10.1128/AAC.42.7.1778 contributor: fullname: Morita – volume: 107 start-page: 193 year: 2004 ident: B24 article-title: Selectable marker genes in transgenic plants: applications, alternatives and biosafety. publication-title: J. Biotechnol. doi: 10.1016/j.jbiotec.2003.10.011 contributor: fullname: Miki – volume: 6 year: 2011 ident: B12 article-title: Aminoglycoside resistance rates, phenotypes, and mechanisms of gram-negative bacteria from infected patients in upper Egypt. publication-title: PLoS One doi: 10.1371/journal.pone.0017224 contributor: fullname: Gad – volume: 311 start-page: 1283 year: 2006 ident: B5 article-title: Toward automatic reconstruction of a highly resolved tree of life. publication-title: Science doi: 10.1126/science.1123061 contributor: fullname: Ciccarelli – volume: 25 start-page: 661 year: 2012 ident: B9 article-title: Adaptive and mutational resistance: role of porins and efflux pumps in drug resistance. publication-title: Clin. Microbiol. Rev. doi: 10.1128/CMR.00043-12 contributor: fullname: Fernandez – volume: 64 start-page: 1841 year: 2007 ident: B17 article-title: Aminoglycoside antibiotics: old drugs and new therapeutic approaches. publication-title: Cell Mol. Life Sci. doi: 10.1007/s00018-007-7034-x contributor: fullname: Hermann – start-page: 1 year: 2016 ident: B27 article-title: Multidrug resistance phenotypes are widespread over different bacterial taxonomic groups thriving in surface water. publication-title: Sci. Total Environ. doi: 10.1016/j.scitotenv.2016.04.062 contributor: fullname: Narciso-da-Rocha – volume: 8 start-page: e1172 year: 2017 ident: B20 article-title: Synergy between active efflux and outer membrane diffusion defines rules of antibiotic permeation into gram-negative bacteria. publication-title: mBio doi: 10.1128/mBio.01172-17 contributor: fullname: Krishnamoorthy – year: 2016 ident: B28 publication-title: Prevalence of Antibiotic Resistance Marker Genes (ARMG) in Selected Environments in Norway. doi: 10.3389/fmicb.2012.00408 contributor: fullname: Nordgård – volume: 28 start-page: 1823 year: 2012 ident: B31 article-title: SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. publication-title: Bioinformatics doi: 10.1093/bioinformatics/bts252 contributor: fullname: Pruesse – volume: 8 start-page: 105 year: 2013 ident: B3 article-title: Aminoglycoside antibiotics in the 21st century. publication-title: ACS Chem. Biol. doi: 10.1021/cb3005116 contributor: fullname: Becker – volume: 5 start-page: D20 year: 2000 ident: B38 article-title: Bacterial resistance to aminoglycosides and beta-lactams: the Tn1331 transposon paradigm. publication-title: Front. Biosci. doi: 10.1093/bioinformatics/btu033 contributor: fullname: Tolmasky – volume: 69 start-page: 4455 year: 2003 ident: B7 article-title: Spread of recombinant DNA by roots and pollen of transgenic potato plants, identified by highly specific biomonitoring using natural transformation of an Acinetobacter sp. publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.69.8.4455-4462.2003 contributor: fullname: de Vries – volume: 117 start-page: 132 year: 2018 ident: B22 article-title: Critical knowledge gaps and research needs related to the environmental dimensions of antibiotic resistance. publication-title: Environ. Int. doi: 10.1016/j.envint.2018.04.041 contributor: fullname: Larsson |
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Snippet | Through a culture-based approach using sludge from drinking water treatment plants, this study reports on the presence of aminoglycoside resistant bacteria at... |
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SubjectTerms | agriculture antimicrobial resistance (AMR) drinking water environmental bacteria Microbiology sludge water treatment |
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Title | Detection of Aminoglycoside Resistant Bacteria in Sludge Samples From Norwegian Drinking Water Treatment Plants |
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