ASSIsT: an automatic SNP scoring tool for in- and outbreeding species
ASSIsT (Automatic SNP ScorIng Tool) is a user-friendly customized pipeline for efficient calling and filtering of SNPs from Illumina Infinium arrays, specifically devised for custom genotyping arrays. Illumina has developed an integrated software for SNP data visualization and inspection called Geno...
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Published in | Bioinformatics (Oxford, England) Vol. 31; no. 23; pp. 3873 - 3874 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
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England
Oxford University Press
01.12.2015
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Abstract | ASSIsT (Automatic SNP ScorIng Tool) is a user-friendly customized pipeline for efficient calling and filtering of SNPs from Illumina Infinium arrays, specifically devised for custom genotyping arrays. Illumina has developed an integrated software for SNP data visualization and inspection called GenomeStudio (GS). ASSIsT builds on GS-derived data and identifies those markers that follow a bi-allelic genetic model and show reliable genotype calls. Moreover, ASSIsT re-edits SNP calls with null alleles or additional SNPs in the probe annealing site. ASSIsT can be employed in the analysis of different population types such as full-sib families and mating schemes used in the plant kingdom (backcross, F1, F2), and unrelated individuals. The final result can be directly exported in the format required by the most common software for genetic mapping and marker-trait association analysis. ASSIsT is developed in Python and runs in Windows and Linux.
The software, example data sets and tutorials are freely available at http://compbiotoolbox.fmach.it/assist/.
eric.vandeweg@wur.nl. |
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AbstractList | ASSIsT (Automatic SNP ScorIng Tool) is a user-friendly customized pipeline for efficient calling and filtering of SNPs from Illumina Infinium arrays, specifically devised for custom genotyping arrays. Illumina has developed an integrated software for SNP data visualization and inspection called GenomeStudio (GS). ASSIsT builds on GS-derived data and identifies those markers that follow a bi-allelic genetic model and show reliable genotype calls. Moreover, ASSIsT re-edits SNP calls with null alleles or additional SNPs in the probe annealing site. ASSIsT can be employed in the analysis of different population types such as full-sib families and mating schemes used in the plant kingdom (backcross, F1, F2), and unrelated individuals. The final result can be directly exported in the format required by the most common software for genetic mapping and marker-trait association analysis. ASSIsT is developed in Python and runs in Windows and Linux.
The software, example data sets and tutorials are freely available at http://compbiotoolbox.fmach.it/assist/.
eric.vandeweg@wur.nl. ASSIsT (Automatic SNP ScorIng Tool) is a user-friendly customized pipeline for efficient calling and filtering of SNPs from Illumina Infinium arrays, specifically devised for custom genotyping arrays. Illumina has developed an integrated software for SNP data visualization and inspection called GenomeStudio® (GS). ASSIsT builds on GS-derived data and identifies those markers that follow a bi-allelic genetic model and show reliable genotype calls. Moreover, ASSIsT re-edits SNP calls with null alleles or additional SNPs in the probe annealing site. ASSIsT can be employed in the analysis of different population types such as full-sib families and mating schemes used in the plant kingdom (backcross, F1, F2), and unrelated individuals. The final result can be directly exported in the format required by the most common software for genetic mapping and marker–trait association analysis. ASSIsT is developed in Python and runs in Windows and Linux. Availability and implementation: The software, example data sets and tutorials are freely available at http://compbiotoolbox.fmach.it/assist/ . Contact: eric.vandeweg@wur.nl UNLABELLEDASSIsT (Automatic SNP ScorIng Tool) is a user-friendly customized pipeline for efficient calling and filtering of SNPs from Illumina Infinium arrays, specifically devised for custom genotyping arrays. Illumina has developed an integrated software for SNP data visualization and inspection called GenomeStudio (GS). ASSIsT builds on GS-derived data and identifies those markers that follow a bi-allelic genetic model and show reliable genotype calls. Moreover, ASSIsT re-edits SNP calls with null alleles or additional SNPs in the probe annealing site. ASSIsT can be employed in the analysis of different population types such as full-sib families and mating schemes used in the plant kingdom (backcross, F1, F2), and unrelated individuals. The final result can be directly exported in the format required by the most common software for genetic mapping and marker-trait association analysis. ASSIsT is developed in Python and runs in Windows and Linux.AVAILABILITY AND IMPLEMENTATIONThe software, example data sets and tutorials are freely available at http://compbiotoolbox.fmach.it/assist/.CONTACTeric.vandeweg@wur.nl. ASSIsT (Automatic SNP ScorIng Tool) is a user-friendly customized pipeline for efficient calling and filtering of SNPs from Illumina Infinium arrays, specifically devised for custom genotyping arrays. Illumina has developed an integrated software for SNP data visualization and inspection called GenomeStudio® (GS).ASSIsT builds on GS derived data and identifies those markers that follow a bi-allelic genetic model and show reliable genotype calls. Moreover, ASSIsT re-edits SNP calls with null alleles or additional SNPs in the probe annealing site. ASSIsT can be employed in the analysis of different population types such as full-sib families and mating schemes used in the plant kingdom (backcross, F1, F2), and unrelated individuals. The final result can be directly exported in the format required by the most common software for genetic mapping and marker-trait association analysis. ASSIsT is developed in Python and runs in Windows and Linux. Abstract ASSIsT (Automatic SNP ScorIng Tool) is a user-friendly customized pipeline for efficient calling and filtering of SNPs from Illumina Infinium arrays, specifically devised for custom genotyping arrays. Illumina has developed an integrated software for SNP data visualization and inspection called GenomeStudio® (GS). ASSIsT builds on GS-derived data and identifies those markers that follow a bi-allelic genetic model and show reliable genotype calls. Moreover, ASSIsT re-edits SNP calls with null alleles or additional SNPs in the probe annealing site. ASSIsT can be employed in the analysis of different population types such as full-sib families and mating schemes used in the plant kingdom (backcross, F1, F2), and unrelated individuals. The final result can be directly exported in the format required by the most common software for genetic mapping and marker–trait association analysis. ASSIsT is developed in Python and runs in Windows and Linux. Availability and implementation: The software, example data sets and tutorials are freely available at http://compbiotoolbox.fmach.it/assist/. Contact: eric.vandeweg@wur.nl |
Author | Bianco, Luca Aranzana, Maria J Costa, Fabrizio Troggio, Michela van de Weg, Eric W Micheletti, Diego Koehorst-van Putten, Herma J J Arús, Pere Di Guardo, Mario Longhi, Sara Velasco, Riccardo |
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Cites_doi | 10.1371/journal.pone.0110377 10.1186/s12864-015-1310-1 10.1007/s11295-013-0679-8 10.1371/journal.pone.0067407 10.1534/g3.114.011023 |
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References | 25886969 - BMC Genomics. 2015;16:155 25303088 - PLoS One. 2014;9(10):e110377 23826289 - PLoS One. 2013;8(6):e67407 25031181 - G3 (Bethesda). 2014 Sep;4(9):1681-7 Kermani (2023020202402640500_btv446-B4) 2006 Bianco (2023020202402640500_btv446-B2) 2014; 9 Pikunova (2023020202402640500_btv446-B5) 2014; 10 Troggio (2023020202402640500_btv446-B6) 2013; 8 Gardner (2023020202402640500_btv446-B3) 2014; 4 Bassil (2023020202402640500_btv446-B1) 2015; 16 |
References_xml | – volume: 9 start-page: e110377 year: 2014 ident: 2023020202402640500_btv446-B2 article-title: Development and validation of a 20K single nucleotide polymorphism (SNP) whole genome genotyping array for apple (Malus × domestica Borkh) publication-title: PLoS One doi: 10.1371/journal.pone.0110377 contributor: fullname: Bianco – volume: 16 start-page: 155 year: 2015 ident: 2023020202402640500_btv446-B1 article-title: Development and preliminary evaluation of a 90K Axiom® SNP array in the allo-octoploid cultivated strawberry Fragaria × ananassa publication-title: BMC Genomics doi: 10.1186/s12864-015-1310-1 contributor: fullname: Bassil – year: 2006 ident: 2023020202402640500_btv446-B4 contributor: fullname: Kermani – volume: 10 start-page: 261 year: 2014 ident: 2023020202402640500_btv446-B5 article-title: ‘Schmidt’s Antonovka’ is identical to ‘Common Antonovka’, an apple cultivar widely used in Russia in the breeding for biotic and abiotic stresses publication-title: Tree Genet. Genom. doi: 10.1007/s11295-013-0679-8 contributor: fullname: Pikunova – volume: 8 start-page: e67407 year: 2013 ident: 2023020202402640500_btv446-B6 article-title: Evaluation of SNP data from the Malus infinium array identifies challenges for genetic analysis of complex genomes of polyploid origin publication-title: PLoS One doi: 10.1371/journal.pone.0067407 contributor: fullname: Troggio – volume: 4 start-page: 1681 year: 2014 ident: 2023020202402640500_btv446-B3 article-title: Fast and cost-effective genetic mapping in apple using next-generation sequencing publication-title: G3 doi: 10.1534/g3.114.011023 contributor: fullname: Gardner |
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SubjectTerms | Alleles Animals Applications Notes Genotyping Techniques - methods Humans Laboratorium voor Plantenveredeling Laboratory of Plant Breeding PBR Biodiversiteit en Genetische Variatie PBR Biodiversity and genetic variation PBR Ornamentals, tissue culture and gene transfer PBR Siergewassen, Tissue Culture Plant Breeding Polymorphism, Single Nucleotide Software WUR PB Biodiversiteit en Genetische Variatie WUR PB Siergewassen, Tissue Culture |
Title | ASSIsT: an automatic SNP scoring tool for in- and outbreeding species |
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