Exploring Rain as Source of Biological Control Agents for Fire Blight on Apple

• Published in: Frontiers in microbiology Vol. 11; p. 199
• Main Authors: Mechan Llontop, Marco E, Hurley, Kelly, Tian, Long, Bernal Galeano, Vivian A, Wildschutte, Hans K, Marine, Sasha C, Yoder, Keith S, Vinatzer, Boris A
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 14.02.2020
• Subjects: biocontrol; fire blight; genome-based identification; Microbiology; rain; UV mutagenesis; rain; genome-based identification; fire blight; biocontrol; UV mutagenesis
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2020.00199

Poor survival on plants can limit the efficacy of Biological Control Agents (BCAs) in the field. Yet bacteria survive in the atmosphere, despite their exposure to high solar radiation and extreme temperatures. If conditions in the atmosphere are similar to, or more extreme than, the environmental conditions on the plant surface, then precipitation may serve as a reservoir of robust BCAs. To test this hypothesis, two hundred and fifty-four rain-borne isolates were screened for inhibition of , the causal agent of fire blight, as well as of other plant pathogenic bacteria, fungi and oomycetes. Two isolates showed strong activity against and other plant pathogenic bacteria, while other isolates showed activity against fungal and oomycete pathogens. Survival assays suggested that the two isolates that inhibited were able to survive on apple blossoms and branches similarly to . Pathogen population size and associated fire blight symptoms were significantly reduced when detached apple blossoms were treated with the two isolates before pathogen inoculation, however, disease reduction on attached blossoms within an orchard was inconsistent. Using whole genome sequencing, the isolates were identified as and , respectively. A UV-mutagenesis screen pointed to a phenazine antibiotic D-alanylgriseoluteic acid synthesis gene cluster as being at the base of the antimicrobial activity of the isolate. Our work reveals the potential of precipitation as an under-explored source of BCAs, whole genome sequencing as an effective approach to precisely identify BCAs, and UV-mutagenesis as a technically simple screen to investigate the genetic basis of BCAs. More field trials are needed to determine the efficacy of the identified BCAs in fire blight control.