Thionein Can Serve as a Reducing Agent for the Methionine Sulfoxide Reductases

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 103; no. 23; pp. 8656 - 8661
• Main Authors: Sagher, Daphna, Brunell, David, Hejtmancik, J. Fielding, Kantorow, Marc, Brot, Nathan, Weissbach, Herbert
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 06.06.2006; National Acad Sciences
• Subjects: Amino acids; Animal cells; Animals; Biochemistry; Biological Sciences; Biotechnology; Cattle; Cells; Dithiothreitol - pharmacology; Edetic Acid - pharmacology; Enzymes; Ergothioneine - metabolism; Ergothioneine - pharmacology; Escherichia coli; Humans; Liver; Liver - enzymology; Metallothionein - metabolism; Methionine Sulfoxide Reductases; Oxidation; Oxidation-Reduction - drug effects; Oxidoreductases - isolation & purification; Oxidoreductases - metabolism; Protein synthesis; Rats; Recombinant Proteins - metabolism; Ribonucleic acid; RNA; Sulfoxides; Thioredoxin; Thioredoxins - metabolism; Ungulates; Zinc; Zinc - metabolism

It has been generally accepted, primarily from studies on methionine sulfoxide reductase (Msr) A, that the biological reducing agent for the members of the Msr family is reduced thioredoxin (Trx), although high levels of DTT can be used as the reductant in vitro. Preliminary experiments using both h...