Cryopreservation: An Overview of Principles and Cell-Specific Considerations

• Published in: Cell transplantation Vol. 30; p. 963689721999617
• Main Authors: Whaley, David, Damyar, Kimia, Witek, Rafal P., Mendoza, Alan, Alexander, Michael, Lakey, Jonathan RT
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA SAGE Publications 2021; Sage Publications Ltd; SAGE Publishing
• Subjects: Alginic acid; Apoptosis; Cell death; Cell survival; Cryopreservation; Dimethyl sulfoxide; Embryo cells; Ethylene glycol; Glycerol; Hematopoietic stem cells; Hepatocytes; Low temperature; Mesenchyme; Oocytes; Osmotic stress; Oxidants; Pancreas; Propylene glycol; Review (Unsolicited); Stem cells; Thawing; low temperature banking; cryoprotectants; freezing; cryopreservation

The origins of low-temperature tissue storage research date back to the late 1800s. Over half a century later, osmotic stress was revealed to be a main contributor to cell death during cryopreservation. Consequently, the addition of cryoprotective agents (CPAs) such as dimethyl sulfoxide (DMSO), gly...