Endocytic uptake of a large array of HPMA copolymers: Elucidation into the dependence on the physicochemical characteristics

• Published in: Journal of controlled release Vol. 143; no. 1; pp. 71 - 79
• Main Authors: Liu, Jihua, Bauer, Hillevi, Callahan, Jon, Kopečková, Pavla, Pan, Huaizhong, Kopeček, Jindřich
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 02.04.2010; Elsevier
• Subjects: Acrylamides - chemistry; Acrylamides - metabolism; Biological and medical sciences; Biological Transport; Cell Line, Tumor; Charge; Chemistry, Pharmaceutical; Chromatography, Gel; Clathrin - metabolism; Drug Carriers; Endocytic uptake; Endocytosis; Endosomes - metabolism; Flow Cytometry; General pharmacology; HPMA copolymer; Humans; Hydrophobic and Hydrophilic Interactions; Intracellular traffickings; Kinetics; Male; Medical sciences; Microscopy, Confocal; Molecular Structure; Molecular Weight; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Physicochemical characteristics; Pinocytosis; Prostatic Neoplasms - metabolism; rab GTP-Binding Proteins - genetics; rab GTP-Binding Proteins - metabolism; Structure-Activity Relationship; Surface Properties; Technology, Pharmaceutical - methods; Transfection; MATC; SEMA; Mn; Charge; DEMA; Mw; CPZ; MAA; Endocytic uptake; PAMAM; P; P-FITC; FITC; MA; Physicochemical characteristics; PEI; FBS; HPMA copolymer; GFLG; Intracellular traffickings; HPMA; RFP; Methacrylamide derivative copolymer; Endocytosis; Pharmaceutical technology; Dependence; Uptake; Physicochemical properties

Endocytic uptake and subcellular trafficking of a large array of HPMA ( N-(2-hydroxypropyl)methacrylamide) based copolymers possessing positively or negatively charged residues, or hydrophobic groups were evaluated by flow cytometry and living cell confocal microscopy in cultured prostate cancer cel...