Protein tracking and detection of protein motion using atomic force microscopy

• Published in: Biophysical journal Vol. 70; no. 5; pp. 2421 - 2431
• Main Authors: Thomson, N.H., Fritz, M., Radmacher, M., Cleveland, J.P., Schmidt, C.F., Hansma, P.K.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.05.1996
• Subjects: Animals; Brain - ultrastructure; Cattle; Fourier Analysis; Freeze Drying; Immunoglobulin G - physiology; Immunoglobulin G - ultrastructure; Microscopy, Atomic Force - instrumentation; Microscopy, Atomic Force - methods; Microtubules - physiology; Microtubules - ultrastructure; Quaternary Ammonium Compounds; Surface-Active Agents; Time Factors; Urease - physiology; Urease - ultrastructure

Height fluctuations over three different proteins, immunoglobulin G, urease, and microtubules, have been measured using an atomic force microscope (AFM) operating in fluid tapping mode. This was achieved by using a protein-tracking system, where the AFM tip was periodically repositioned above a sing...