Lysozyme gene treatment in testosterone induced benign prostate hyperplasia rat model and comparasion of its' effectiveness with botulinum toxin injection
To compare the effects and histopathological changes of botulinum neurotoxin type A and lysozyme gene injections into prostate tissue within a testosterone induced benign prostate hyperplasia rat model. 40 male Wistar rats were randomized into four Groups. Group-1: Control, Group-2: Testosterone rep...
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Published in | International Brazilian Journal of Urology Vol. 43; no. 6; pp. 1167 - 1175 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Brazil
Sociedade Brasileira de Urologia
01.11.2017
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Abstract | To compare the effects and histopathological changes of botulinum neurotoxin type A and lysozyme gene injections into prostate tissue within a testosterone induced benign prostate hyperplasia rat model.
40 male Wistar rats were randomized into four Groups. Group-1: Control, Group-2: Testosterone replacement, Group-3: Testosterone+botulinum neurotoxin type A, Group-4: Testosterone+plazmid DNA/liposome complex.
Estimated prostate volume of the testosterone injected Groups were higher than the control (p <0.05). Actual prostate weight of the testosterone injected Groups was higher than the control Group (p <0.05). Testosterone undecanoate increased the prostate weight by 39%. Botulinum neurotoxin type A treatment led to an estimated prostate volume and actual prostate weights decreased up to 32.5% in rats leading to prostate apoptosis. Lysozyme gene treatment led to an estimated prostate volume and actual prostate weights decrease up to 38.7%.
Lysozyme gene and botulinum neurotoxin type A treatments for prostate volume decreasing effect have been verified in the present study that could be anew modality of treatment in prostatic benign hyperplasia that needs to be verified in large randomized human experimental studies. |
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AbstractList | ABSTRACT Objectives: To compare the effects and histopathological changes of botulinum neurotoxin type A and lysozyme gene injections into prostate tissue within a testosterone induced benign prostate hyperplasia rat model. Materials and Methods: 40 male Wistar rats were randomized into four Groups. Group-1: Control, Group-2: Testosterone replacement, Group-3: Testosterone+botulinum neurotoxin type A, Group-4: Testosterone+plazmid DNA/liposome complex. Results: Estimated prostate volume of the testosterone injected Groups were higher than the control (p <0.05). Actual prostate weight of the testosterone injected Groups was higher than the control Group (p <0.05). Testosterone undecanoate increased the prostate weight by 39%. Botulinum neurotoxin type A treatment led to an estimated prostate volume and actual prostate weights decreased up to 32.5% in rats leading to prostate apoptosis. Lysozyme gene treatment led to an estimated prostate volume and actual prostate weights decrease up to 38.7%. Conclusion: Lysozyme gene and botulinum neurotoxin type A treatments for prostate volume decreasing effect have been verified in the present study that could be anew modality of treatment in prostatic benign hyperplasia that needs to be verified in large randomized human experimental studies. To compare the effects and histopathological changes of botulinum neurotoxin type A and lysozyme gene injections into prostate tissue within a testosterone induced benign prostate hyperplasia rat model. 40 male Wistar rats were randomized into four Groups. Group-1: Control, Group-2: Testosterone replacement, Group-3: Testosterone+botulinum neurotoxin type A, Group-4: Testosterone+plazmid DNA/liposome complex. Estimated prostate volume of the testosterone injected Groups were higher than the control (p <0.05). Actual prostate weight of the testosterone injected Groups was higher than the control Group (p <0.05). Testosterone undecanoate increased the prostate weight by 39%. Botulinum neurotoxin type A treatment led to an estimated prostate volume and actual prostate weights decreased up to 32.5% in rats leading to prostate apoptosis. Lysozyme gene treatment led to an estimated prostate volume and actual prostate weights decrease up to 38.7%. Lysozyme gene and botulinum neurotoxin type A treatments for prostate volume decreasing effect have been verified in the present study that could be anew modality of treatment in prostatic benign hyperplasia that needs to be verified in large randomized human experimental studies. |
Author | Darici, Hakan Koşar, Alim Koşar, Pinar Aslan Onaran, İbrahim Ergün, Osman |
Author_xml | – sequence: 1 givenname: Osman surname: Ergün fullname: Ergün, Osman organization: Department of Urology, Konya Training and Research Hospital, Konya, Turkey – sequence: 2 givenname: Pinar Aslan surname: Koşar fullname: Koşar, Pinar Aslan organization: Department of Medical Biology and Genetic, Süleyman Demirel University, Isparta, Turkey – sequence: 3 givenname: İbrahim surname: Onaran fullname: Onaran, İbrahim organization: Department of Medical Biology and Genetic, Süleyman Demirel University, Isparta, Turkey – sequence: 4 givenname: Hakan surname: Darici fullname: Darici, Hakan organization: Deparment of Histology and Embryology, Süleyman Demirel University, Isparta, Turkey – sequence: 5 givenname: Alim surname: Koşar fullname: Koşar, Alim organization: Department of Urology, Süleyman Demirel University, Isparta, Turkey |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/28727388$$D View this record in MEDLINE/PubMed |
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Keywords | Botulinum Toxins Prostatic Hyperplasia Testosterone |
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Notes | CONFLICT OF INTEREST None declared. |
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SubjectTerms | Animals Botulinum Toxins Botulinum Toxins, Type A - therapeutic use Disease Models, Animal Genetic Therapy - methods Male Muramidase - genetics Original Prostatic Hyperplasia Prostatic Hyperplasia - chemically induced Prostatic Hyperplasia - drug therapy Rats Rats, Wistar Testosterone |
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Title | Lysozyme gene treatment in testosterone induced benign prostate hyperplasia rat model and comparasion of its' effectiveness with botulinum toxin injection |
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