The Listeria monocytogenes Core-Genome Sequence Typer (LmCGST): a bioinformatic pipeline for molecular characterization with next-generation sequence data

• Published in: BMC microbiology Vol. 15; no. 1; p. 224
• Main Authors: Pightling, Arthur W., Petronella, Nicholas, Pagotto, Franco
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 22.10.2015; BioMed Central
• Subjects: Analysis; Chromosomes; Computational Biology - methods; Electrophoresis; Genetic aspects; Genomes; High-Throughput Nucleotide Sequencing; Listeria; Listeria monocytogenes - classification; Listeria monocytogenes - genetics; Methods; Molecular Typing - methods; Organisms; Phylogenetics; Phylogeny; Proteins; Sequence Analysis, DNA; Single nucleotide polymorphisms

Next-generation sequencing provides a powerful means of molecular characterization. However, methods such as single-nucleotide polymorphism detection or whole-chromosome sequence analysis are computationally expensive, prone to errors, and are still less accessible than traditional typing methods. H...