Ultra-Sensitive HIV-1 Latency Viral Outgrowth Assays Using Humanized Mice

• Published in: Frontiers in immunology Vol. 9; p. 344
• Main Authors: Schmitt, Kimberly, Akkina, Ramesh
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 05.03.2018
• Subjects: Acquired Immunodeficiency Syndrome - immunology; Animals; Biological Assay - methods; CD4-Positive T-Lymphocytes - immunology; CD4-Positive T-Lymphocytes - transplantation; CD4-Positive T-Lymphocytes - virology; comparison of mVOA with humanized mouse-based viral outgrowth assay; comparison of quantitative viral outgrowth assays with humanized mouse-based viral outgrowth assay; Heterografts; HIV-1 - physiology; HIV-1 latent viral outgrowth assay using humanized mice; humanized mouse-based HIV-1 latency outgrowth assay; Humans; Immunology; Mice; non-human primate-based latent simian immunodeficiency viral outgrowth assay; sensitivity of humanized mouse-based viral outgrowth assay over mVOA; Virus Activation - immunology; Virus Latency - immunology; comparison of quantitative viral outgrowth assays with humanized mouse-based viral outgrowth assay; ultra-sensitive HIV-1 latent viral outgrowth assay in hu-mice; sensitivity of humanized mouse-based viral outgrowth assay over mVOA; humanized mouse-based HIV-1 latency outgrowth assay; non-human primate-based latent simian immunodeficiency viral outgrowth assay; mouse-based HIV-1 viral outgrowth assays; comparison of mVOA with humanized mouse-based viral outgrowth assay; HIV-1 latent viral outgrowth assay using humanized mice

In the current quest for a complete cure for HIV/AIDS, highly sensitive HIV-1 latency detection methods are critical to verify full viral eradication. Until now, the quantitative viral outgrowth assays (qVOA) have been the gold standard for assessing latent HIV-1 viral burden. However, these assays...