Recombinant protein expression in Escherichia coli: advances and challenges

• Published in: Frontiers in microbiology Vol. 5; p. 172
• Main Authors: Rosano, Germán L, Ceccarelli, Eduardo A
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 17.04.2014
• Subjects: affinity tags; E. coli expression strains; Escherichia coli; expression plasmid; Inclusion Bodies; Microbiology; recombinant protein production; E. coli expression strains; expression plasmid; inclusion bodies; recombinant protein expression; affinity tags; Escherichia coli

Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of...