Recombinant protein expression in Escherichia coli: advances and challenges
Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of...
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Published in | Frontiers in microbiology Vol. 5; p. 172 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
17.04.2014
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Subjects | |
Online Access | Get full text |
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Summary: | Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of heterologous proteins, such as a vast catalog of expression plasmids, a great number of engineered strains and many cultivation strategies. We review the different approaches for the synthesis of recombinant proteins in E. coli and discuss recent progress in this ever-growing field. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1 Reviewed by: Jose M. Bruno-Barcena, North Carolina State University, USA; Thomas Schweder, Ernst-Moritz-Arndt-Universität Greifswald, Germany This article was submitted to Microbiotechnology, Ecotoxicology and Bioremediation, a section of the journal Frontiers in Microbiology. Edited by: Peter Neubauer, Technische Universität Berlin, Germany |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2014.00172 |