Functional characterization of Musca glutamate- and GABA-gated chloride channels expressed independently and coexpressed in Xenopus oocytes

• Published in: Insect molecular biology Vol. 15; no. 6; pp. 773 - 783
• Main Authors: Eguchi, Y., Ihara, M., Ochi, E., Shibata, Y., Matsuda, K., Fushiki, S., Sugama, H., Hamasaki, Y., Niwa, H., Wada, M., Ozoe, F., Ozoe, Y.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.12.2006
• Subjects: Animals; Antiparasitic Agents - pharmacology; Chloride Channel Agonists; Chloride Channels - antagonists & inhibitors; Chloride Channels - chemistry; Chloride Channels - metabolism; Cloning, Molecular; coexpression; DNA, Complementary - metabolism; Dose-Response Relationship, Drug; GABA Agonists - pharmacology; GABA Antagonists - pharmacology; GABA-gated chloride channels; gamma-Aminobutyric Acid - metabolism; gamma-Aminobutyric Acid - pharmacology; Gene Expression; glutamate-gated chloride channels; Glutamates - pharmacology; Ion Channel Gating - drug effects; Lindane - pharmacology; Musca; Musca domestica; Muscidae - metabolism; Oocytes - drug effects; Oocytes - metabolism; Picrotoxin - analogs & derivatives; Picrotoxin - pharmacology; Protein Structure, Quaternary - drug effects; Pyrazoles - pharmacology; receptor; RNA, Complementary - metabolism; Xenopus laevis

Ligand‐gated chloride channels (LGICs) are important targets for insecticides and parasiticides. Genes encoding subunits of two LGICs, a glutamate‐gated chloride channel (MdGluCl‐α) and a γ‐aminobutyric acid (GABA)‐gated chloride channel (MdRdl), were cloned from house‐flies (Musca domestica L.). Th...