PHD3-VHL axis controls HIV-2 infection through oxygen-dependent hydroxylation and degradation of Vpx

• Published in: PLoS pathogens Vol. 21; no. 6; p. e1013241
• Main Authors: Miyakawa, Kei, Tanaka, Kiho, Ino, Yoko, Kimura, Yayoi, Kameya, Taichi, Mizukoshi, Fuminori, Nishi, Mayuko, Yokoyama, Masaru, Nakabayashi, Jun, Nomaguchi, Masako, Sato, Hironori, Kimura, Hirokazu, Akari, Hirofumi, Miura, Tomoyuki, Takaoka, Akinori, Hasegawa, Hideki, Matano, Tetsuro, Minamishima, Yoji Andrew, Ryo, Akihide
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 16.06.2025; Public Library of Science (PLoS)
• Subjects: Analysis; Biology and Life Sciences; Crosslinked polymers; Health aspects; HEK293 Cells; HIV Infections - metabolism; HIV Infections - virology; HIV-2 - metabolism; Humans; Hydroxylation; Hypoxia-Inducible Factor-Proline Dioxygenases - genetics; Hypoxia-Inducible Factor-Proline Dioxygenases - metabolism; Infection control; Macrophages - metabolism; Macrophages - virology; Medicine and Health Sciences; Methods; Oxygen - metabolism; Proteolysis; Research and Analysis Methods; Ubiquitin-proteasome system; Viral proteins; Viral Regulatory and Accessory Proteins - genetics; Viral Regulatory and Accessory Proteins - metabolism; Von Hippel-Lindau Tumor Suppressor Protein - genetics; Von Hippel-Lindau Tumor Suppressor Protein - metabolism; Japan

HIV-2 viral protein X (Vpx) plays a pivotal role in antagonizing the host restriction factors, including SAMHD1 and components of the HUSH complex, to facilitate viral replication. However, the regulatory mechanisms controlling Vpx stability remain unclear. In this study, we identify the von Hippel–...