Development of a lateral flow immunoassay strip for rapid detection of IgG antibody against SARS-CoV-2 virus

• Published in: Analyst (London) Vol. 145; no. 15; pp. 5345 - 5352
• Main Authors: Wen, Tian, Huang, Chao, Shi, Feng-Juan, Zeng, Xiao-Yan, Lu, Tian, Ding, Shou-Nian, Jiao, Yong-Jun
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 07.08.2020
• Subjects: Antibodies, Viral - blood; Antigens; Betacoronavirus - isolation & purification; Betacoronavirus - metabolism; Conjugation; Coronavirus Infections - diagnosis; Coronavirus Infections - virology; Coronavirus Nucleocapsid Proteins; Coronaviruses; Coupling (molecular); COVID-19; Gold; Gold - chemistry; Humans; IgG antibody; Immunoassay; Immunoassay - methods; Immunoglobulin G - blood; Immunoglobulin M - blood; Metal Nanoparticles - chemistry; Nanoparticles; Nucleocapsid Proteins - immunology; Pandemics; Phosphoproteins; Physicians; Pneumonia, Viral - diagnosis; Pneumonia, Viral - virology; Point-of-Care Systems; Public health; Reproducibility of Results; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Strip; Viral diseases; Viruses

The ongoing worldwide SARS-CoV-2 epidemic clearly has a tremendous influence on public health. Molecular detection based on oral swabs was used for confirmation of SARS-CoV-2 infection. However, high false negative rates were reported. We describe here the development of a point-of-care (POC) serolo...