CYP1B1 expression promotes the proangiogenic phenotype of endothelium through decreased intracellular oxidative stress and thrombospondin-2 expression

• Published in: Blood Vol. 113; no. 3; pp. 744 - 754
• Main Authors: Tang, Yixin, Scheef, Elizabeth A., Wang, Shoujian, Sorenson, Christine M., Marcus, Craig B., Jefcoate, Colin R., Sheibani, Nader
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.01.2009; American Society of Hematology
• Series: Vascular Biology
• Subjects: Animals; Antioxidants - pharmacology; Aryl Hydrocarbon Hydroxylases - genetics; Aryl Hydrocarbon Hydroxylases - metabolism; Blotting, Western; Cell Movement; Cytochrome P-450 CYP1B1; Endothelium, Vascular - drug effects; Endothelium, Vascular - metabolism; Endothelium, Vascular - pathology; Gene Expression; Gene Expression Regulation; Humans; Immunohistochemistry; In Situ Nick-End Labeling; Lipid Peroxidation - drug effects; Mice; Mice, Mutant Strains; Microscopy, Fluorescence; Neovascularization, Pathologic - genetics; Neovascularization, Pathologic - metabolism; Oxidative Stress - drug effects; Oxidative Stress - physiology; Phenotype; Reactive Oxygen Species - metabolism; Retinal Vessels - drug effects; Retinal Vessels - metabolism; Retinal Vessels - pathology; RNA, Small Interfering; Thrombospondins - biosynthesis; Vascular Biology

Reactive species derived from cell oxygenation processes play an important role in vascular homeostasis and the pathogenesis of many diseases including retinopathy of prematurity. We show that CYP1B1-deficient (CYP1B1−/−) mice fail to elicit a neovascular response during oxygen-induced ischemic reti...