Development of immunocompatible pluripotent stem cells via CRISPR-based human leukocyte antigen engineering

• Published in: Experimental & molecular medicine Vol. 51; no. 1; pp. 1 - 11
• Main Authors: Jang, Yeonsue, Choi, Jinhyeok, Park, Narae, Kang, Jaewoo, Kim, Myungshin, Kim, Yonggoo, Ju, Ji Hyeon
• Format: Journal Article
• Language: English
• Published: United States Springer Nature B.V 07.01.2019; Nature Publishing Group UK; Nature Publishing Group; 생화학분자생물학회
• Subjects: Animals; Antigens; Antisera; Cell lines; Cells, Cultured; CRISPR; CRISPR-Cas Systems; Graft rejection; Histocompatibility; Histocompatibility antigen HLA; HLA Antigens - genetics; Humans; Immunogenicity; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - immunology; Induced Pluripotent Stem Cells - transplantation; Male; Mice; Mice, Nude; Pluripotency; Stem cell transplantation; Stem cells; 생화학

Pluripotent stem cell transplantation is a promising regenerative strategy for treating intractable diseases. However, securing human leukocyte antigen (HLA)-matched donor stem cells is extremely difficult. The traditional approach for generating such cells is to establish homozygous pluripotent ste...