Coexpression of type 2 immune targets in sputum-derived epithelial and dendritic cells from asthmatic subjects

• Published in: Journal of allergy and clinical immunology Vol. 136; no. 3; pp. 619 - 627.e5
• Main Authors: Bleck, Bertram, Kazeros, Angeliki, Bakal, Keren, Garcia-Medina, Lymaris, Adams, Alexandra, Liu, Mengling, Lee, Richard A., Tse, Doris B., Chiu, Amanda, Grunig, Gabriele, Egan, John P., Reibman, Joan
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.2015; Elsevier Limited
• Subjects: Adult; Allergy and Immunology; Antigens, CD1 - genetics; Antigens, CD1 - immunology; Asthma; Asthma - genetics; Asthma - immunology; Asthma - pathology; B7-1 Antigen - genetics; B7-1 Antigen - immunology; B7-2 Antigen - genetics; B7-2 Antigen - immunology; bronchial epithelial cells; Case-Control Studies; CCL17; Cell Communication; Chemokine CCL17 - genetics; Chemokine CCL17 - immunology; Cytokines; Dendritic cells; Dendritic Cells - immunology; Dendritic Cells - metabolism; Dendritic Cells - pathology; Epithelial Cells - immunology; Epithelial Cells - metabolism; Epithelial Cells - pathology; Ethnicity; Female; Gene Expression; Gene Expression Profiling; Human subjects; Humans; IL-33; Inflammation; Interleukin-17 - genetics; Interleukin-17 - immunology; Interleukin-33 - genetics; Interleukin-33 - immunology; Laboratories; Male; Middle Aged; OX40 ligand; OX40 Ligand - genetics; OX40 Ligand - immunology; Phosphoprotein Phosphatases - genetics; Phosphoprotein Phosphatases - immunology; Signal Transduction - genetics; Signal Transduction - immunology; sputum; Sputum - cytology; Studies; thymic stromal lymphopoietin; SSC; bronchial epithelial cells; TSLP; OX40L; sputum; FACS; IQR; IL-33; CK-7; EGFR; Asthma; Ct; thymic stromal lymphopoietin; dendritic cells; OX40 ligand; sDC; FSC; qPCR; CCL17; GAPDH; sHBEC; DC; Gyceraldehyde-3-phosphate dehydrogenase; Epidermal growth factor receptor; Dendritic cell; Sputum-derived human bronchial epithelial cell; Cytokeratin-7; Quantitative PCR; Side scatter; Fluorescence-activated cell sorting; Sputum-derived myeloid type 1 dendritic cell; Interquartile range; Cycle threshold; Forward scatter

Noninvasive sputum sampling has enabled the identification of biomarkers in asthmatic patients. Studies of discrete cell populations in sputum can enhance measurements compared with whole sputum in which changes in rare cells and cell-cell interactions can be masked. We sought to enrich for sputum-d...