Neuregulin-1 converts reactive astrocytes toward oligodendrocyte lineage cells via upregulating the PI3K-AKT-mTOR pathway to repair spinal cord injury

• Published in: Biomedicine & pharmacotherapy Vol. 134; p. 111168
• Main Authors: Ding, Zhenfei, Dai, Ce, Zhong, Lin, Liu, Rui, Gao, Weilu, Zhang, Hui, Yin, Zongsheng
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.02.2021
• Subjects: Animals; Astrocytes - drug effects; Astrocytes - enzymology; Astrocytes - pathology; Cell Lineage; Cell Transdifferentiation - drug effects; Cells, Cultured; Disease Models, Animal; ErbB Receptors - metabolism; Female; Myelin Sheath - metabolism; Neuregulin-1; Neuregulin-1 - pharmacology; Oligodendrocyte; Oligodendroglia - drug effects; Oligodendroglia - enzymology; Oligodendroglia - pathology; Phosphatidylinositol 3-Kinase - metabolism; Proto-Oncogene Proteins c-akt - metabolism; Rats; Rats, Sprague-Dawley; Reactive astrocyte; Remyelination; Signal Transduction; Spinal Cord - drug effects; Spinal Cord - enzymology; Spinal Cord - pathology; Spinal Cord Injuries - drug therapy; Spinal Cord Injuries - enzymology; Spinal Cord Injuries - pathology; Spinal cord injury; TOR Serine-Threonine Kinases - metabolism; Tumor Necrosis Factor-alpha - pharmacology; df; OPC; IHC; LFB; WB; Spinal cord injury; Neuregulin-1; PI3K; Oligodendrocyte; NF-H; mTOR; CCK-8; MBP; IF; Remyelination; PBS; EGF; qRT-PCR; DAPI; Nrg1; GFAP; TNF-α; SCI; ErbBI; GAPDH; Reactive astrocyte

[Display omitted] •TNF-α treatment induces dedifferentiation of primary rat spinal cord astrocytes.•Nrg1 converts reactive astrocytes toward oligodendrocyte lineage cells in vitro and in vivo.•Nrg1 upregulates PI3K-AKT-mTOR signaling pathway in astrocytes.•Nrg1 repairs SCI by enhancing remyelination...