New Approach to Antifolate Treatment of Certain Cancers as Demonstrated in Tissue Culture

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 72; no. 10; pp. 4018 - 4022
• Main Authors: Halpern, Richard M., Halpern, Barbara C., Clark, Brian R., Ashe, Hilary, Hardy, Dorothy N., Jenkinson, Pamela Y., Chou, Shao-Chia, Smith, Roberts A.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 01.10.1975; National Acad Sciences
• Subjects: Adults; Animals; Carcinoma, Ehrlich Tumor - drug therapy; Cell Division - drug effects; Cell growth; Cells, Cultured; Cultured cells; Epithelial cells; Female; Fibroblasts - drug effects; Folic acid antagonists; Formyltetrahydrofolates; Humans; Leucovorin - therapeutic use; Leukemia; Leukemia L1210 - drug therapy; Leukemia, Myeloid - drug therapy; Liver; Liver - drug effects; Methotrexate - pharmacology; Methotrexate - therapeutic use; Mice; Neoplasms, Experimental - drug therapy; Rats; Tetrahydrofolates; Tetrahydrofolates - pharmacology; Tetrahydrofolates - therapeutic use; Tissue culture techniques

The selective toxicity of antifolates for a variety of cancers can be improved, as illustrated by the combined administration of N5-methyltetrahydrofolate and methotrexate in tissue culture. When a variety of neoplastic cell types characterized by a deficiency of vitamin B12-dependent N5-methyltetra...