Towards Scarless Wound Healing : A Comparison of Protein Expression between Human, Adult and Foetal Fibroblasts

• Published in: BioMed research international Vol. 2014; no. 2014; pp. 1 - 10
• Main Authors: Ho, Sonia, Marçal, Helder, Foster, Leslie John Ray
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Puplishing Corporation 01.01.2014; Hindawi Publishing Corporation; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Adult; Cicatrix - genetics; Cicatrix - metabolism; Collagen; Electrophoresis, Gel, Two-Dimensional; Extracellular matrix; Fetus - metabolism; Fibroblasts; Fibroblasts - metabolism; Gene expression; Gene Expression Regulation, Developmental; Humans; Protein Biosynthesis - genetics; Protein expression; Protein Processing, Post-Translational; Proteins; Proteomics; RNA, Messenger - metabolism; Skin; Studies; Tissue engineering; Wound healing; Wound Healing - genetics; Australia
• DOI: 10.1155/2014/676493
• ISSN: 2314-6133

Proteins from human adult and foetal fibroblast cell lines were compared, focusing on those involved in wound healing. Proteins were separated through two-dimensional gel electrophoresis (2DE). Differences in protein spot intensity between the lineages were quantified through 3D gel scanning densitometry. Selected protein spots were excised, subjected to tryptic digests, prior to separation using HPLC with a linear ion trap mass spectrometer, and identified. Protein maps representing the proteomes from adult and foetal fibroblasts showed similar distributions but revealed differences in expression levels. Heat shock cognate 71 kDA protein, Tubulin alpha-1A chain, actin cytoplasmic-1, and neuron cytoplasmic protein were all expressed in significantly higher concentrations by foetal fibroblasts, nearly double those observed for their adult counterparts. Fructose bisphosphate aldolase A, Cofilin-1, Peroxiredoxin-1, Lactotransferrin Galectin-1, Profilin-1, and Calreticulin were expressed at comparatively higher concentrations by the adult fibroblasts. Significant differences in the expression levels of some proteins in human adult and foetal fibroblasts correlated with known differences in wound healing behaviour. This data may assist in the development of technologies to promote scarless wound healing and better functional tissue repair and regeneration.