RNA-Sequencing for profiling goat milk transcriptome in colostrum and mature milk

• Published in: BMC veterinary research Vol. 12; no. 1; p. 264
• Main Authors: Crisà, Alessandra, Ferrè, Fabrizio, Chillemi, Giovanni, Moioli, Bianca
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 25.11.2016; BioMed Central
• Subjects: Animals; cell death; cell proliferation; Colostrum; Colostrum - metabolism; cytokines; Female; Gene Expression Profiling; gene expression regulation; Genetic aspects; Genetic transcription; genome; glycolysis; goat milk; Goat's milk; goats; Goats - genetics; immune response; Lactation; lymphocyte proliferation; milk; Milk - metabolism; morphogenesis; oligosaccharides; Physiological aspects; RNA sequencing; Sequence Analysis, RNA; sequence homology; Transcriptome; Goat milk; Lactation; RNA-seq; Milk somatic cells; Colostrum; Oligosaccharides; Transcriptomic profile

In this work we aimed at sequencing and assembling the goat milk transcriptome corresponding at colostrum and 120 days of lactation. To reconstruct transcripts we used both the genome as reference, and a de novo assembly approach. Additionally, we aimed at identifying the differentially expressed ge...