Evaluation of safe utilization of l-threonine for supplementation in healthy adults: a randomized double blind controlled trial

• Published in: Amino acids Vol. 57; no. 1; p. 30
• Main Authors: Matsumoto, Hideki, Miura, Naoki, Naito, Masaki, Elango, Rajavel
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 27.05.2025; Springer Nature B.V; Springer
• Subjects: Adult; Adults; Adverse events; Amino acid; Amino acids; Analytical Chemistry; Anthropometry; aspartate transaminase; Biochemical Engineering; Biochemistry; Biomedical and Life Sciences; blood; Blood pressure; Blood Pressure - drug effects; butylate; Clinical trials; Creatine; Creatine kinase; Creatine Kinase - blood; Cross-Over Studies; Dietary intake; Dietary supplements; Dietary Supplements - adverse effects; Double-Blind Method; Evaluation; Food intake; Healthy Volunteers; Heart rate; Human study; Humans; Kinases; Life Sciences; Male; males; Metabolites; Middle Aged; Neurobiology; no observed adverse effect level; NOAEL; Original; Original Article; Parameters; Physical examinations; placebos; Proteomics; Randomized controlled clinical trial; Threonine; Threonine - administration & dosage; Threonine - adverse effects; Threonine - blood; Randomized controlled clinical trial; Amino acid; Threonine; Human study; NOAEL
• DOI: 10.1007/s00726-025-03461-6
• ISSN: 1438-2199; 0939-4451

l -threonine is used in dietary supplements and nutritional products ingested by healthy consumers. The objective of this study was to determine in a randomized double blind controlled clinical trial the safety and tolerability of l -threonine used as graded doses in supplements for 4 weeks. Healthy male adults (age 42.9) ingested randomly placebo or different doses of L-threonine (0, 3, 6, 9, 12 g/day) for 4 weeks using a crossover design. At the end of supplementation period, the subjects visited the clinic for medical examination, anthropometric parameter measurements, blood sampling for biochemical tests including amino acid concentrations in plasma, measurement of blood pressure and heart rate, and dietary intake evaluation. Adverse events were recorded all along the trial. None of the anthropometric parameters measured, dietary intake and the biochemical parameters were affected by l -threonine supplementation except a non-specific minor increase in plasma aspartate amino transferase and creatine kinase which was measured in the group supplemented with 9 g l -threonine per day but not with the 12 g per day dose. Also, the concentration of L-threonine as well as the concentration of its metabolite L-2-amino butylate were found to be increased in plasma after supplementation with 6, 9, 12 g/day L-threonine. The moderate and mild adverse events were found to occur at random. All symptoms disappeared during the supplementation period despite continuous L-threonine supplementation. These results of this study indicate a no-observed-adverse-effect-level (NOAEL) value for L-threonine to be 12 g/day in healthy adult males. This study was registered at jRCT as jRCT1050230137.