Expression of recombinant Arabian camel lactoferricin-related peptide in Pichia pastoris and its antimicrobial identification

• Published in: Journal of the science of food and agriculture Vol. 96; no. 2; pp. 569 - 575
• Main Authors: Chahardooli, Mahmood, Niazi, Ali, Aram, Farzaneh, Sohrabi, Seyyed Mohsen
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 30.01.2016; John Wiley and Sons, Limited
• Subjects: Amino Acid Sequence; Animals; Anti-Infective Agents - pharmacology; Antiinfectives and antibacterials; antimicrobial activity; Bacillus subtilis; Bacillus subtilis - drug effects; Base Sequence; camel lactoferricin; Camels; Camelus; Candida albicans; Candida albicans - drug effects; Culture; Drug Stability; E coli; Escherichia coli; Escherichia coli - drug effects; Gene Expression; Hot Temperature; Lactoferrin - biosynthesis; Lactoferrin - chemistry; Lactoferrin - genetics; Lactoferrin - pharmacology; Methanol; Methyl alcohol; Microbial Sensitivity Tests; Molecular Sequence Data; Peptides; Peptides - genetics; Peptides - pharmacology; Pichia - metabolism; Pichia pastoris; Pseudomonas aeruginosa; Pseudomonas aeruginosa - drug effects; Recombinant; Recombinant Proteins - biosynthesis; Recombinant Proteins - genetics; Recombinant Proteins - pharmacology; Saudi Arabia; Sequence Alignment; Staphylococcus aureus; Staphylococcus aureus - drug effects; Staphylococcus infections; thermal stability; camel lactoferricin; thermal stability; Pichia pastoris; antimicrobial activity
• ISSN: 0022-5142; 1097-0010
• DOI: 10.1002/jsfa.7125

BACKGROUND Lactoferricin (LFcin) is a strong cationic peptide released from the N‐terminus of lactoferrin by gastric pepsin digestion. LFcin has some important properties, including high antimicrobial activity. To date, lactoferricins have been isolated and characterised from various animal species, but not from camel. The aim of this study was to characterise and express recombinant camel lactoferricin (LFcinC) in Pichia pastoris and investigate its antimicrobial activity. RESULTS After methanol induction, LFcinC was expressed and secreted into a culture broth medium and the results determined by concentrated supernatant culture medium showed high antimicrobial activity against the following microorganisms: Escherichia coli PTCC 1330 (ATCC 8739), Staphylococcus aureus PTCC 1112 (ATCC 6538), Pseudomonas aeruginosa PTCC 1074 (ATCC 9027), Bacillus subtilis PTCC 1023 (ATCC 6633), and Candida albicans PTCC 5027 (ATCC 10231). Thermal stability was clarified with antibacterial activity against Escherichia coli PTCC 1330 (ATCC 8739). CONCLUSION Results confirmed that camel lactoferricin had suitable antimicrobial activity and its production by Pichia pastoris can be used for recombinant production. © 2015 Society of Chemical Industry