Evaluation of Surgical Tissue From Patients with Crohn's Disease for the Presence of Mycobacterium avium Subspecies paratuberculosis DNA by In Situ Hybridization and Nested Polymerase Chain Reaction

• Published in: Inflammatory bowel diseases Vol. 11; no. 2; pp. 116 - 125
• Main Authors: Romero, Claudia, Hamdi, Amal, Valentine, John F., Naser, Saleh A.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford University Press 01.02.2005; Lippincott Williams & Wilkins, Inc
• Subjects: Adult; Aged; Case-Control Studies; Crohn Disease - microbiology; Crohn Disease - pathology; Crohn Disease - surgery; Crohn's disease; DNA, Bacterial - analysis; Female; fluorescence in situ hybridization; Humans; In Situ Hybridization; Inflammatory bowel disease; Male; Middle Aged; Mycobacterium avium; Mycobacterium avium subsp paratuberculosis; Mycobacterium avium subsp. paratuberculosis - genetics; Mycobacterium avium subsp. paratuberculosis - isolation & purification; Mycobacterium avium subsp. paratuberculosis - pathogenicity; PCR; Polymerase Chain Reaction; fluorescence in situ hybridization; Crohn's disease; subsp; PCR

Crohn's disease (CD) is a chronic inflammatory bowel disease (IBD) with tissue granuloma and histopathological alteration that resembles aspects in tuberculosis, leprosy, and paratuberculosis. Mycobacterium avium subsp paratuberculosis (MAP) is the causative agent of paratuberculosis, with a su...