Direct production of 4‐hydroxybenzoic acid from cellulose using cellulase‐displaying Pichia pastoris

• Published in: Biotechnology and Bioengineering Vol. 120; no. 4; pp. 1097 - 1107
• Main Authors: Inokuma, Kentaro, Miyamoto, Shunya, Morinaga, Kohei, Kobayashi, Yuma, Kumokita, Ryota, Bamba, Takahiro, Ito, Yoichiro, Kondo, Akihiko, Hasunuma, Tomohisa
• Format: Journal Article
• Language: English
• Published: United States Wiley 01.04.2023; Wiley Subscription Services, Inc
• Subjects: 4‐hydroxybenzoic acid; Aromatic compounds; Cell surface; Cellobiohydrolase; Cellulase; Cellulase - metabolism; Cellulolytic enzymes; Cellulose; Cellulose - metabolism; Fermentation; Glucose; Glucose - metabolism; Glucosidase; Glycosylphosphatidylinositol; Phosphoric acid; Pichia pastoris; Pyruvic acid; Saccharomyces cerevisiae; Saccharomyces cerevisiae - metabolism; simultaneous saccharification and fermentation; Yeast; yeast surface display; simultaneous saccharification and fermentation; Pichia pastoris; 4-hydroxybenzoic acid; yeast surface display; cellulase

4‐hydroxybenzoic acid (4‐HBA) is an industrially important aromatic compound, and there is an urgent need to establish a bioprocess to produce this compound in a sustainable and environmentally friendly manner from renewable feedstocks such as cellulosic biomass. Here, we developed a bioprocess to d...