The calcium‐independent protein kinase C participates in an early process of CD3/CD28‐mediated induction of thymocyte apoptosis

• Published in: Immunology Vol. 101; no. 3; pp. 309 - 315
• Main Authors: Asada, A., Zhao, Y., Komano, H., Kuwata, T., Mukai, M., Fujita, K., Tozawa, Y., Iseki, R., Tian, H., Sato, K., Motegi, Y., Suzuki, R., Yokoyama, M., Iwata, M.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.11.2000; Wiley Subscription Services, Inc; Blackwell Science Inc
• Subjects: Animals; Apoptosis - immunology; calcium; Calcium - physiology; CD28 antigen; CD28 Antigens - immunology; CD3 antigen; CD3 Complex - immunology; CD4-Positive T-Lymphocytes - immunology; CD8-Positive T-Lymphocytes - immunology; Cell Culture Techniques; Clonal Anergy - immunology; Isoenzymes - metabolism; Lymphocyte Activation - immunology; Mice; Mice, Inbred BALB C; Mice, Transgenic; Original; Protein Kinase C - immunology; Protein Kinase C - metabolism; T-Lymphocyte Subsets - enzymology; T-Lymphocyte Subsets - immunology

Summary Thymocyte negative selection eliminates self‐reactive clones and involves both a T‐cell receptor (TCR)/CD3‐mediated signal and a costimulatory signal, which can be delivered via CD28. Anti‐CD3/anti‐CD28‐triggered apoptosis in isolated CD4+CD8+ thymocytes in vitro provides a basic model for n...