Relationship of Liver X Receptors α and Endoglin Levels in Serum and Placenta with Preeclampsia

• Published in: PloS one Vol. 11; no. 10; p. e0163742
• Main Authors: Wang, Jing, Dong, Xing, Wu, Hong-yan, Wu, Nan, Zhang, Xue-jun, Wang, Xin, Shang, Li-xin
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 13.10.2016; Public Library of Science (PLoS)
• Subjects: Adult; Biology and Life Sciences; Cholesterol; Colorectal cancer; Correlation analysis; Endoglin; Endoglin - analysis; Endoglin - blood; Endoglin - genetics; Enzyme-linked immunosorbent assay; Female; Gene Expression Regulation; Gynecology; Humans; Immunohistochemistry; Liver; Liver X receptors; Liver X Receptors - analysis; Liver X Receptors - blood; Liver X Receptors - genetics; Medicine and Health Sciences; Metabolism; Obstetrics; Pathogenesis; Patients; Placenta; Placenta - metabolism; Placenta - pathology; Pre-eclampsia; Pre-Eclampsia - blood; Pre-Eclampsia - diagnosis; Pre-Eclampsia - genetics; Pre-Eclampsia - pathology; Predictions; Preeclampsia; Pregnancy; Receptors; Research and Analysis Methods; Substance abuse treatment; Young Adult; Beijing China; China
• DOI: 10.1371/journal.pone.0163742
• ISSN: 1932-6203

Liver X receptor alpha (LXRα) and endoglin have been postulated to play roles in trophoblast invasion and lipid metabolic disturbances. However, the relationship between LXRα and endoglin levels in serum and placenta of patients with preeclampsia remains poorly understood. The objective of this study was to identify correlations between LXRα, endoglin and preeclampsia and provide new feasible methods of clinical prediction and treatment for preeclampsia. We enrolled 45 patients with preeclampsia (24 with moderate preeclampsia and 21 with severe preeclampsia) and 15 normal pregnant women (control group) who were admitted to the Department of Obstetrics of the General Hospital of Beijing Command between October 2012 and July 2013 in this study. Serum and placental LXRα and endoglin levels were analyzed by enzyme-linked immunosorbent assay, real-time quantitative PCR, tissue microarray and immunohistochemistry. Serum and placental LXRα and endoglin levels were significantly higher in patients with preeclampsia than those in control group (P<0.05, each). Moreover, patients with severe preeclampsia displayed significantly higher LXRα and endoglin levels than those with moderate preeclampsia (P<0.05, each). The LXRα sensitivity, specificity and positive and negative predictive values were 66.00%, 80.00%, 89.19% and 48.48%, respectively, while those of endoglin levels were 62.00%, 85.00%, 91.18% and 47.22%, respectively. LXRα and endoglin levels in serum and placenta from patients with preeclampsia were positively correlated (serum: r = 0.486, P<0.01; placenta: r = 0.569, P<0.01). Elevated LXRα and endoglin levels may be associated with preeclampsia pathogenesis and development and could be used as potential predictors for this disorder.