Monooxygenase, a Novel Beta-Cypermethrin Degrading Enzyme from Streptomyces sp

• Published in: PloS one Vol. 8; no. 9; p. e75450
• Main Authors: Chen, Shaohua, Lin, Qingsheng, Xiao, Ying, Deng, Yinyue, Chang, Changqing, Zhong, Guohua, Hu, Meiying, Zhang, Lian-Hui
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 30.09.2013; Public Library of Science (PLoS)
• Subjects: Aluminum; Bacillus cereus; Base Sequence; Biodegradation; Biology; Cloning; Contamination; Copper; Cypermethrin; Degradation; Detoxification; Environmental effects; Environmental Pollution - prevention & control; Environmental protection; Enzymatic activity; Enzyme activity; Enzymes; Homogeneity; Hydrogen-Ion Concentration; Hydrolase; Hydroxylation; Insecticides; Insecticides - metabolism; Ionization; Iron; Mass spectrometry; Mass spectroscopy; Metals, Heavy - pharmacology; Methods; Microorganisms; Mixed Function Oxygenases - chemistry; Mixed Function Oxygenases - genetics; Mixed Function Oxygenases - isolation & purification; Mixed Function Oxygenases - metabolism; Molecular Sequence Data; Molecular structure; Molecular Weight; Monooxygenase; Natural resources; Pesticide pollution; Pesticides; pH effects; Proteins; Public concern; Pyrethrins - metabolism; Pyrethroids; Sequence Analysis, DNA; Soil contamination; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sphingobium; Streptomyces; Streptomyces - enzymology; Substrates; Temperature; Terminal protein; Toxicity; China; Singapore

The widely used insecticide beta-cypermethrin has become a public concern because of its environmental contamination and toxic effects on mammals. In this study, a novel beta-cypermethrin degrading enzyme designated as CMO was purified to apparent homogeneity from a Streptomyces sp. isolate capable...