Defining the subcellular interface of nanoparticles by live-cell imaging

• Published in: PloS one Vol. 8; no. 4; p. e62018
• Main Authors: Hemmerich, Peter H, von Mikecz, Anna H
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 26.04.2013; Public Library of Science (PLoS)
• Subjects: Active transport; Animals; Biology; Biophysics; Cell Survival; Cells (biology); Chemistry; Confocal microscopy; Correlation; Cytoplasm; Diffusion; Dynamin; Endocytosis; Fluorescence; Fluorescence Recovery After Photobleaching; Fluorescence spectroscopy; Gene expression; HeLa Cells; Humans; Hydrazones - metabolism; Imaging techniques; Intracellular; Materials Science; Mice; Microenvironments; Microscopy; Mitochondria; Morphology; Nanomaterials; Nanoparticles; Nanoparticles - chemistry; Nanoparticles - ultrastructure; Nanotechnology; NIH 3T3 Cells; Nuclear reactions; Nuclear transport; Optical Imaging - methods; Organelles; Particle motion; Particle Size; Photobleaching; Polystyrene; Polystyrene resins; Polystyrenes - metabolism; Proteins; Quantum dots; Recovery (Medical); Silica; Silicon dioxide; Silicon Dioxide - chemistry; Spectroscopy; Subcellular Fractions - metabolism; Surface chemistry; Translocation

Understanding of nanoparticle-bio-interactions within living cells requires knowledge about the dynamic behavior of nanomaterials during their cellular uptake, intracellular traffic and mutual reactions with cell organelles. Here, we introduce a protocol of combined kinetic imaging techniques that e...