Direct Comparison of Flow-FISH and qPCR as Diagnostic Tests for Telomere Length Measurement in Humans

• Published in: PloS one Vol. 9; no. 11; p. e113747
• Main Authors: Gutierrez-Rodrigues, Fernanda, Santana-Lemos, Bárbara A., Scheucher, Priscila S., Alves-Paiva, Raquel M., Calado, Rodrigo T.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 19.11.2014; Public Library of Science (PLoS)
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Anemia; Anemia, Aplastic - metabolism; Anemia, Aplastic - pathology; Biology; Biology and Life Sciences; Bone marrow; Breast cancer; Cardiovascular disease; Child; Child, Preschool; Chromosomes; Coefficient of variation; Correlation; Correlation analysis; Cytometry; Deoxyribonucleic acid; Diagnostic systems; Diagnostic tests; DNA; Dyskeratosis Congenita - metabolism; Dyskeratosis Congenita - pathology; Erosion mechanisms; Female; Flow; Flow cytometry; Fluorescence; Fluorescence in situ hybridization; Genetic testing; Humans; Hybridization; Idiopathic Pulmonary Fibrosis - metabolism; Idiopathic Pulmonary Fibrosis - pathology; In Situ Hybridization, Fluorescence - methods; Infant; Infant, Newborn; Internal medicine; Laboratories; Leukocytes; Leukocytes - cytology; Leukocytes - metabolism; Male; Medical diagnosis; Medicine; Medicine and Health Sciences; Methods; Middle Aged; Mutation; Patients; Pulmonary fibrosis; Real-Time Polymerase Chain Reaction - methods; Sensitivity; Sensitivity and Specificity; Studies; Telomerase; Telomere - metabolism; Telomere Shortening - physiology; Telomeres; White blood cells; Young Adult; Brazil

Telomere length measurement is an essential test for the diagnosis of telomeropathies, which are caused by excessive telomere erosion. Commonly used methods are terminal restriction fragment (TRF) analysis by Southern blot, fluorescence in situ hybridization coupled with flow cytometry (flow-FISH),...