Downregulation of endothelial nitric oxide synthase (eNOS) and endothelin-1 (ET-1) in a co-culture system with human stimulated X-linked CGD neutrophils

• Published in: PloS one Vol. 15; no. 4; p. e0230665
• Main Authors: Nakamura-Utsunomiya, Akari, Tsumura, Miyuki, Okada, Satoshi, Kawaguchi, Hiroshi, Kobayashi, Masao
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 2020; Public Library of Science (PLoS)
• Subjects: Biology and Life Sciences; Calcimycin; Calcium; Calcium ionophores; Cell culture; Chronic granulomatous disease; Culture; Endothelial cells; Endothelin 1; Gene expression; Growth factors; Health sciences; Hydrogen peroxide; Leukocytes (neutrophilic); Medicine and Health Sciences; mRNA; Neutrophils; NF-κB protein; Nitric oxide; Nitric-oxide synthase; Oxidative stress; Pediatrics; Phagocytes; Phosphorylation; Physical Sciences; Polymerase chain reaction; Proteins; Research and Analysis Methods; Umbilical vein; University graduates; United States > US; Japan

Phagocytes in patients with chronic granulomatous disease (CGD) do not generate reactive oxidative species (ROS), whereas nitric oxide (NO) production is increased in response to the calcium ionophore A23187 in CGD phagocytes compared with healthy phagocytes. Recently, patients with X-linked CGD (X-...