Potential Role of Notch Signalling in CD34+ Chronic Myeloid Leukaemia Cells: Cross-Talk between Notch and BCR-ABL

• Published in: PloS one Vol. 10; no. 4; p. e0123016
• Main Authors: Aljedai, Abdullah, Buckle, Anne-Marie, Hiwarkar, Prashant, Syed, Farhatullah
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 07.04.2015; Public Library of Science (PLoS)
• Subjects: Abl gene; Abl protein; Antagonism; Antigens, CD34 - metabolism; Apoptosis - drug effects; Basic Helix-Loop-Helix Transcription Factors - genetics; Basic Helix-Loop-Helix Transcription Factors - metabolism; BCR gene; BCR protein; BCR-ABL protein; BCR-ABL1 gene; Blotting, Western; Bone marrow; CD34 antigen; Cell Cycle - drug effects; Cell lines; Cell Proliferation - drug effects; Cell self-renewal; Cell survival; Cells (biology); Chronic myeloid leukemia; Drosophila; Enzyme inhibitors; Fusion protein; Fusion Proteins, bcr-abl - genetics; Fusion Proteins, bcr-abl - metabolism; Gene expression; Histone deacetylase; Homeodomain Proteins - genetics; Homeodomain Proteins - metabolism; Humans; Imatinib; Imatinib Mesylate - therapeutic use; Inhibition; Inhibitors; Leukemia; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - drug therapy; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - genetics; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - metabolism; Neoplastic Stem Cells - metabolism; Notch protein; Notch1 protein; Notch2 protein; Patients; Protein Kinase Inhibitors - therapeutic use; Protein-tyrosine kinase; Real-Time Polymerase Chain Reaction; Receptor, Notch1 - genetics; Receptor, Notch1 - metabolism; Receptor, Notch2 - genetics; Receptor, Notch2 - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; Signal transduction; Signaling; Stem cells; Transcription Factor HES-1; Tumor Cells, Cultured; Tyrosine; Valproic acid

Notch signalling is critical for haemopoietic stem cell (HSC) self-renewal and survival. The role of Notch signalling has been reported recently in chronic myeloid leukaemia (CML) - a stem cell disease characterized by BCR-ABL tyrosine kinase activation. Therefore, we studied the relationship betwee...