Antigenic diversity among Helicobacter pylori vacuolating toxins

• Published in: Infection and immunity Vol. 69; no. 7; pp. 4329 - 4336
• Main Authors: VINION-DUBIEL, Arlene D, MCCLAIN, Mark S, PING CAO, MERNAUGH, Raymond L, COVER, Timothy L
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.07.2001
• Subjects: Animals; Antibodies, Monoclonal - biosynthesis; Antigenic Variation - immunology; Antigens, Bacterial - immunology; Bacterial diseases; Bacterial diseases of the digestive system and abdomen; Bacterial Proteins - immunology; Bacterial Toxins - immunology; Bacteriology; Biological and medical sciences; Epitope Mapping; Epitopes, B-Lymphocyte - immunology; Female; Fundamental and applied biological sciences. Psychology; Helicobacter pylori; Helicobacter pylori - immunology; Human bacterial diseases; Infectious diseases; Medical sciences; Mice; Mice, Inbred BALB C; Microbiology; Molecular Pathogenesis; Neutralization Tests; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Recombinant Fusion Proteins - immunology; VacA protein; vacuolating toxin; vacuolating toxins; Immunization; Immunoglobulins; Spirillales; Secretion; Rodentia; Diversity; Spirillaceae; Monoclonal antibody; Protein A; Strain; Toxin; Vertebrata; Generation; Type; Mammalia; Antigenicity; Helicobacter pylori; Mouse; Bacteria; Epithelial cell

Helicobacter pylori vacuolating cytotoxin (VacA) is a secreted protein that induces vacuolation of epithelial cells. To study VacA structure and function, we immunized mice with purified type s1-m1 VacA from H. pylori strain 60190 and generated a panel of 10 immunoglobulin G1kappa anti-VacA monoclon...