Differential responses of Coffea arabica L. leaves and roots to chemically induced systemic acquired resistance

• Published in: Genome Vol. 49; no. 12; pp. 1594 - 1605
• Main Authors: De Nardi, B, Dreos, R, Del Terra, L, Martellossi, C, Asquini, E, Tornincasa, P, Gasperini, D, Pacchioni, B, Rathinavelu, R, Pallavicini, A
• Format: Journal Article
• Language: English
• Published: Canada NRC Research Press 01.12.2006; Canadian Science Publishing NRC Research Press
• Subjects: Agricultural commodities; benzo(1,2,3)thiadiazole-7-carbothioic acid-s-methyl ester; Binding sites; BTH; cDNA microarray; cell wall components; Chemical compounds; Coffea - drug effects; Coffea - genetics; Coffea - immunology; Coffea arabica; complementary DNA; Developing countries; DNA libraries; esters; Expressed Sequence Tags; expression génique; feuille; Gene expression; gene expression regulation; Gene Expression Regulation, Plant - drug effects; Genes, Plant - physiology; Genetic aspects; genetic markers; Genetic research; genomics; Grants; Immunity, Innate - drug effects; Immunity, Innate - genetics; induced resistance; LDCs; leaf; leaves; Life sciences; Medical treatment; messenger RNA; microarray d'ADNc; microarray technology; Nucleotide sequence; Oligonucleotide Array Sequence Analysis; oxidation; pathogenesis-related proteins; Plant Leaves - drug effects; Plant Leaves - genetics; Plant Roots - drug effects; Plant Roots - genetics; Proteins; racine; resistance mechanisms; Reverse Transcriptase Polymerase Chain Reaction; root; roots; SAR; Sequence Analysis, DNA; systemic acquired resistance; Technical support; Thiadiazoles - pharmacology
• ISSN: 0831-2796; 1480-3321
• DOI: 10.1139/g06-125

Coffea arabica is susceptible to several pests and diseases, some of which affect the leaves and roots. Systemic acquired resistance (SAR) is the main defence mechanism activated in plants in response to pathogen attack. Here, we report the effects of benzo(1,2,3)thiadiazole-7-carbothioic acid-s-methyl ester (BTH), a SAR chemical inducer, on the expression profile of C. arabica. Two cDNA libraries were constructed from the mRNA isolated from leaves and embryonic roots to create 1587 nonredundant expressed sequence tags (ESTs). We developed a cDNA microarray containing 1506 ESTs from the leaves and embryonic roots, and 48 NBS-LRR (nucleotide-binding site leucine-rich repeat) gene fragments derived from 2 specific genomic libraries. Competitive hybridization between untreated and BTH-treated leaves resulted in 55 genes that were significantly overexpressed and 16 genes that were significantly underexpressed. In the roots, 37 and 42 genes were over and underexpressed, respectively. A general shift in metabolism from housekeeping to defence occurred in the leaves and roots after BTH treatment. We observed a systemic increase in pathogenesis-related protein synthesis, in the oxidative burst, and in the cell wall strengthening processes. Moreover, responses in the roots and leaves varied significantly.