Different Ezh2-Containing Complexes Target Methylation of Histone H1 or Nucleosomal Histone H3

• Published in: Molecular cell Vol. 14; no. 2; pp. 183 - 193
• Main Authors: Kuzmichev, Andrei, Jenuwein, Thomas, Tempst, Paul, Reinberg, Danny
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 23.04.2004
• Subjects: Antibodies, Monoclonal - metabolism; Binding Sites; Blotting, Western; DNA-Binding Proteins; Electrophoresis, Polyacrylamide Gel; Enhancer of Zeste Homolog 2 Protein; Gene Expression Regulation, Enzymologic; Gene Targeting; Genes, Reporter; Genetic Vectors; HeLa Cells; Histones - genetics; Histones - metabolism; Humans; Methylation; Methyltransferases - metabolism; Models, Biological; Nucleosomes - chemistry; Nucleosomes - metabolism; Point Mutation; Polycomb Repressive Complex 2; Precipitin Tests; Protein Isoforms - chemistry; Protein Isoforms - genetics; Protein Isoforms - isolation & purification; Protein Isoforms - metabolism; Protein Processing, Post-Translational; Proteins - chemistry; Proteins - isolation & purification; Proteins - metabolism; Recombinant Proteins - metabolism; Repressor Proteins - chemistry; Repressor Proteins - genetics; Repressor Proteins - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Substrate Specificity; Transcription Factors

Human Enhancer of Zeste homolog (Ezh2) is a histone lysine methyltransferase (HKMT) associated with transcriptional repression. Ezh2 is present in several distinct complexes, one of which, PRC2, we characterized previously. Here we report an additional Ezh2 complex, PRC3. We show that the Ezh2 compl...