Functional Characterization of the Mannitol Promoter of Pseudomonas fluorescens DSM 50106 and Its Application for a Mannitol-Inducible Expression System for Pseudomonas putida KT2440

• Published in: PloS one Vol. 10; no. 7; p. e0133248
• Main Authors: Hoffmann, Jana, Altenbuchner, Josef
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 24.07.2015; Public Library of Science (PLoS)
• Subjects: Activation; Bacteria; Base Sequence; Binding Sites; Data processing; Dehydrogenases; Deoxyribonuclease; Deoxyribonucleic acid; DNA; DNA-directed RNA polymerase; E coli; Electrophoretic mobility; Escherichia coli; Footprinting; Gene Expression; Gene Expression Regulation, Bacterial - drug effects; Gene Order; Genes, Reporter; Genetic Vectors - genetics; Kinases; Mannitol; Mannitol - pharmacology; Metabolism; Molecular Sequence Data; Mutants; Operon; Promoter Regions, Genetic; Protein Binding; Protein expression; Proteins; Pseudomonas fluorescens; Pseudomonas fluorescens - genetics; Pseudomonas fluorescens - metabolism; Pseudomonas putida; Pseudomonas putida - genetics; Pseudomonas putida - metabolism; Reporter gene; Repressor Proteins - metabolism; Ribonucleic acid; RNA; Stress response; Studies; Transcription activation

A new pBBR1MCS-2-derived vector containing the Pseudomonas fluorescens DSM10506 mannitol promoter PmtlE and mtlR encoding its AraC/XylS type transcriptional activator was constructed and optimized for low basal expression. Mannitol, arabitol, and glucitol-inducible gene expression was demonstrated w...